Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950009505> ?p ?o ?g. }
- W2950009505 abstract "Abstract The intensification of the poultry industry over the last sixty years facilitated the evolution of increased virulence and vaccine breaks in Marek’s disease virus (MDV-1). Full genome sequences are essential for understanding why and how this evolution occurred, but what is known about genome-wide variation in MDV comes from laboratory culture. To rectify this, we developed methods for obtaining high quality genome sequences directly from field samples without the need for sequence-based enrichment strategies prior to sequencing. We applied this to the first characterization of MDV-1 genomes from the field, without prior culture. These viruses were collected from vaccinated hosts that acquired naturally circulating field strains of MDV-1, in the absence of a disease outbreak. This reflects the current issue afflicting the poultry industry, where virulent field strains continue to circulate despite vaccination, and can remain undetected due to the lack of overt disease symptoms. We found that viral genomes from adjacent field sites had high levels of overall DNA identity, and despite strong evidence of purifying selection, had coding variations in proteins associated with virulence and manipulation of host immunity. Our methods empower ecological field surveillance, make it possible to determine the basis of viral virulence and vaccine breaks, and can be used to obtain full genomes from clinical samples of other large DNA viruses, known and unknown." @default.
- W2950009505 created "2019-06-27" @default.
- W2950009505 creator A5000354894 @default.
- W2950009505 creator A5016055308 @default.
- W2950009505 creator A5042223836 @default.
- W2950009505 creator A5043624938 @default.
- W2950009505 creator A5053336398 @default.
- W2950009505 creator A5064443693 @default.
- W2950009505 creator A5067345495 @default.
- W2950009505 creator A5069217184 @default.
- W2950009505 creator A5077441742 @default.
- W2950009505 creator A5081536128 @default.
- W2950009505 date "2016-09-01" @default.
- W2950009505 modified "2023-10-14" @default.
- W2950009505 title "DNA from dust: comparative genomics of large DNA viruses in field surveillance samples" @default.
- W2950009505 cites W111466360 @default.
- W2950009505 cites W1502391241 @default.
- W2950009505 cites W1525734744 @default.
- W2950009505 cites W1535122834 @default.
- W2950009505 cites W1540296073 @default.
- W2950009505 cites W1574060875 @default.
- W2950009505 cites W1575349704 @default.
- W2950009505 cites W1603246164 @default.
- W2950009505 cites W1647161740 @default.
- W2950009505 cites W1773931763 @default.
- W2950009505 cites W1810528030 @default.
- W2950009505 cites W1902745641 @default.
- W2950009505 cites W1907205561 @default.
- W2950009505 cites W1975556749 @default.
- W2950009505 cites W1982166284 @default.
- W2950009505 cites W1989855913 @default.
- W2950009505 cites W1990733515 @default.
- W2950009505 cites W1993095739 @default.
- W2950009505 cites W1995252109 @default.
- W2950009505 cites W2003078790 @default.
- W2950009505 cites W2003702509 @default.
- W2950009505 cites W2013931988 @default.
- W2950009505 cites W2020188264 @default.
- W2950009505 cites W2020247323 @default.
- W2950009505 cites W2022291864 @default.
- W2950009505 cites W2026604862 @default.
- W2950009505 cites W2027266940 @default.
- W2950009505 cites W2029641872 @default.
- W2950009505 cites W2029836083 @default.
- W2950009505 cites W2031432943 @default.
- W2950009505 cites W2035073629 @default.
- W2950009505 cites W2038574803 @default.
- W2950009505 cites W2040756306 @default.
- W2950009505 cites W2045944192 @default.
- W2950009505 cites W2050122265 @default.
- W2950009505 cites W2051051370 @default.
- W2950009505 cites W2054187786 @default.
- W2950009505 cites W2055200060 @default.
- W2950009505 cites W2058529119 @default.
- W2950009505 cites W2059924844 @default.
- W2950009505 cites W2060185103 @default.
- W2950009505 cites W2060857332 @default.
- W2950009505 cites W2061800646 @default.
- W2950009505 cites W2063509062 @default.
- W2950009505 cites W2065216220 @default.
- W2950009505 cites W2065280713 @default.
- W2950009505 cites W2070429838 @default.
- W2950009505 cites W2075350111 @default.
- W2950009505 cites W2076176349 @default.
- W2950009505 cites W2076628448 @default.
- W2950009505 cites W2077227332 @default.
- W2950009505 cites W2085910659 @default.
- W2950009505 cites W2088688381 @default.
- W2950009505 cites W2094212509 @default.
- W2950009505 cites W2094743336 @default.
- W2950009505 cites W2097706568 @default.
- W2950009505 cites W2100772193 @default.
- W2950009505 cites W2106578986 @default.
- W2950009505 cites W2107760817 @default.
- W2950009505 cites W2109795498 @default.
- W2950009505 cites W2111899369 @default.
- W2950009505 cites W2111965996 @default.
- W2950009505 cites W2113600043 @default.
- W2950009505 cites W2114883999 @default.
- W2950009505 cites W2120642606 @default.
- W2950009505 cites W2124842612 @default.
- W2950009505 cites W2127774996 @default.
- W2950009505 cites W2128060969 @default.
- W2950009505 cites W2131407420 @default.
- W2950009505 cites W2136129244 @default.
- W2950009505 cites W2136715270 @default.
- W2950009505 cites W2138590306 @default.
- W2950009505 cites W2141327598 @default.
- W2950009505 cites W2141691008 @default.
- W2950009505 cites W2144064375 @default.
- W2950009505 cites W2144956282 @default.
- W2950009505 cites W2152207030 @default.
- W2950009505 cites W2155943701 @default.
- W2950009505 cites W2159518657 @default.
- W2950009505 cites W2160114307 @default.
- W2950009505 cites W2171820897 @default.
- W2950009505 cites W2233674177 @default.
- W2950009505 cites W2298571966 @default.
- W2950009505 cites W2614378976 @default.
- W2950009505 cites W4236236547 @default.