FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950099772> ?p ?o ?g. }

• W2950099772 abstract "Abstract The architectural organization of chromatin can play an important role in genome regulation by affecting the mobility of molecules within its surroundings via binding interactions and molecular crowding. The diffusion of molecules at specific locations in the nucleus can be studied by Fluorescence Correlation Spectroscopy (FCS), a well-established technique based on the analysis of fluorescence intensity fluctuations detected in a confocal observation volume. However, detecting subtle variations of mobility between different chromatin regions remains challenging with currently-available FCS methods. Here we introduce a method that samples multiple positions by slowly scanning the FCS observation volume across the nucleus. Analyzing the data in short time segments, we preserve the high temporal resolution of single-point FCS while probing different nuclear regions in the same cell. Using the intensity level of the probe (or a DNA marker) as a reference, we efficiently sort the FCS segments into different populations and obtain average correlation functions that are associated to different chromatin regions. This sorting and averaging strategy renders the method statistically robust while preserving the observation of intranuclear variations of mobility. Using this approach, we quantified diffusion of monomeric GFP in high versus low chromatin density regions. We found that GFP mobility was reduced in heterochromatin, especially within perinucleolar heterochromatin. Moreover, we found that modulation of chromatin compaction by ATP depletion, or treatment with solution of different osmolarity, differentially-affected the ratio of diffusion in both regions. Then, we used the approach to probe the mobility of estrogen receptor-α (ER) in the vicinity of an integrated multicopy prolactin gene array. Finally, we discussed the coupling of this method with stimulated emission depletion (STED)-FCS, for performing FCS at sub-diffraction spatial scales." @default.
• W2950099772 created "2019-06-27" @default.
• W2950099772 creator A5004363698 @default.
• W2950099772 creator A5005143315 @default.
• W2950099772 creator A5022450636 @default.
• W2950099772 creator A5040316453 @default.
• W2950099772 creator A5054509809 @default.
• W2950099772 creator A5070135589 @default.
• W2950099772 date "2018-10-25" @default.
• W2950099772 modified "2023-10-01" @default.
• W2950099772 title "Measuring mobility in chromatin by intensity sorted FCS" @default.
• W2950099772 cites W1491569850 @default.
• W2950099772 cites W1635552303 @default.
• W2950099772 cites W1833483642 @default.
• W2950099772 cites W1930766016 @default.
• W2950099772 cites W1975210552 @default.
• W2950099772 cites W1979245454 @default.
• W2950099772 cites W1981277012 @default.
• W2950099772 cites W1981765336 @default.
• W2950099772 cites W1981916955 @default.
• W2950099772 cites W1990956469 @default.
• W2950099772 cites W1995029094 @default.
• W2950099772 cites W2003777386 @default.
• W2950099772 cites W2020504063 @default.
• W2950099772 cites W2022393754 @default.
• W2950099772 cites W2025721001 @default.
• W2950099772 cites W2027823711 @default.
• W2950099772 cites W2032265312 @default.
• W2950099772 cites W2036152181 @default.
• W2950099772 cites W2036853573 @default.
• W2950099772 cites W2037403230 @default.
• W2950099772 cites W2043216443 @default.
• W2950099772 cites W2046549939 @default.
• W2950099772 cites W2047784187 @default.
• W2950099772 cites W2050484267 @default.
• W2950099772 cites W2052461863 @default.
• W2950099772 cites W2061638897 @default.
• W2950099772 cites W2073196054 @default.
• W2950099772 cites W2077919955 @default.
• W2950099772 cites W2086475019 @default.
• W2950099772 cites W2088832076 @default.
• W2950099772 cites W2096594220 @default.
• W2950099772 cites W2099183701 @default.
• W2950099772 cites W2100390617 @default.
• W2950099772 cites W2103650945 @default.
• W2950099772 cites W2106888275 @default.
• W2950099772 cites W2107557989 @default.
• W2950099772 cites W2108027073 @default.
• W2950099772 cites W2122354859 @default.
• W2950099772 cites W2126652495 @default.
• W2950099772 cites W2129103509 @default.
• W2950099772 cites W2155323783 @default.
• W2950099772 cites W2156040683 @default.
• W2950099772 cites W2260121977 @default.
• W2950099772 cites W2571411546 @default.
• W2950099772 cites W2585367253 @default.
• W2950099772 cites W2586691160 @default.
• W2950099772 cites W2694038906 @default.
• W2950099772 cites W2729283896 @default.
• W2950099772 cites W2888709156 @default.
• W2950099772 doi "https://doi.org/10.1101/452540" @default.
• W2950099772 hasPublicationYear "2018" @default.
• W2950099772 type Work @default.
• W2950099772 sameAs 2950099772 @default.
• W2950099772 citedByCount "0" @default.
• W2950099772 crossrefType "posted-content" @default.
• W2950099772 hasAuthorship W2950099772A5004363698 @default.
• W2950099772 hasAuthorship W2950099772A5005143315 @default.
• W2950099772 hasAuthorship W2950099772A5022450636 @default.
• W2950099772 hasAuthorship W2950099772A5040316453 @default.
• W2950099772 hasAuthorship W2950099772A5054509809 @default.
• W2950099772 hasAuthorship W2950099772A5070135589 @default.
• W2950099772 hasBestOaLocation W29500997721 @default.
• W2950099772 hasConcept C103435993 @default.
• W2950099772 hasConcept C120665830 @default.
• W2950099772 hasConcept C121332964 @default.
• W2950099772 hasConcept C12554922 @default.
• W2950099772 hasConcept C136009344 @default.
• W2950099772 hasConcept C144618238 @default.
• W2950099772 hasConcept C185592680 @default.
• W2950099772 hasConcept C186060115 @default.
• W2950099772 hasConcept C2780723820 @default.
• W2950099772 hasConcept C54355233 @default.
• W2950099772 hasConcept C552990157 @default.
• W2950099772 hasConcept C83640560 @default.
• W2950099772 hasConcept C86803240 @default.
• W2950099772 hasConcept C91881484 @default.
• W2950099772 hasConcept C95444343 @default.
• W2950099772 hasConceptScore W2950099772C103435993 @default.
• W2950099772 hasConceptScore W2950099772C120665830 @default.
• W2950099772 hasConceptScore W2950099772C121332964 @default.
• W2950099772 hasConceptScore W2950099772C12554922 @default.
• W2950099772 hasConceptScore W2950099772C136009344 @default.
• W2950099772 hasConceptScore W2950099772C144618238 @default.
• W2950099772 hasConceptScore W2950099772C185592680 @default.
• W2950099772 hasConceptScore W2950099772C186060115 @default.
• W2950099772 hasConceptScore W2950099772C2780723820 @default.
• W2950099772 hasConceptScore W2950099772C54355233 @default.
• W2950099772 hasConceptScore W2950099772C552990157 @default.
• W2950099772 hasConceptScore W2950099772C83640560 @default.

• next