Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950121979> ?p ?o ?g. }
- W2950121979 abstract "The genus Fusarium contains more than 300 species, most of which are plant pathogens. Appropriate molecular tools for accurately and rapidly describing temporal and spatial shifts in Fusarium communities would be useful for the development of control strategies. Here, we present a new Fusarium-specific primer pair targeting the translation elongation factor 1-α (EF1α) gene with amplicons of ~430 bp, suitable for MiSeq metabarcoding sequencing. Mock Fusarium communities were used to evaluate its resolution and to optimize read filtering and downstream analyses. The use of the DADA2 pipeline coupled with operational taxonomic unit (OTU) picking at 98% similarity cut-off significantly increased the accuracy of read filtering. Building a phylogenetic tree using a manually curated database as a reference allowed taxonomic assignment at the species or species-complex level. This methodology was tested on soil and maize residue samples collected from crop fields. Up to 18 Fusarium OTUs, belonging to 17 species and 8 species complexes, were obtained, with F. oxysporum being the most abundant species in soil samples, while F. graminearum and F. avenaceum were the most abundant in maize residues. We demonstrated the high performance of this workflow which could be further used for profiling Fusarium species composition and dynamics during the cultivation cycle." @default.
- W2950121979 created "2019-06-27" @default.
- W2950121979 creator A5017214673 @default.
- W2950121979 creator A5039418019 @default.
- W2950121979 creator A5046336339 @default.
- W2950121979 creator A5087545886 @default.
- W2950121979 date "2019-06-10" @default.
- W2950121979 modified "2023-10-18" @default.
- W2950121979 title "A novel metabarcoding approach to investigate Fusarium species composition in soil and plant samples" @default.
- W2950121979 cites W1155498079 @default.
- W2950121979 cites W1562049809 @default.
- W2950121979 cites W1580699817 @default.
- W2950121979 cites W1591658601 @default.
- W2950121979 cites W1635003312 @default.
- W2950121979 cites W1967370468 @default.
- W2950121979 cites W1974598127 @default.
- W2950121979 cites W1975736726 @default.
- W2950121979 cites W1976901402 @default.
- W2950121979 cites W1981986562 @default.
- W2950121979 cites W1987039289 @default.
- W2950121979 cites W1989268853 @default.
- W2950121979 cites W1994184910 @default.
- W2950121979 cites W1996513377 @default.
- W2950121979 cites W2002669173 @default.
- W2950121979 cites W2004204906 @default.
- W2950121979 cites W2005469146 @default.
- W2950121979 cites W2020325178 @default.
- W2950121979 cites W2026414158 @default.
- W2950121979 cites W2031103309 @default.
- W2950121979 cites W2031318705 @default.
- W2950121979 cites W2041188107 @default.
- W2950121979 cites W2051201019 @default.
- W2950121979 cites W2051478751 @default.
- W2950121979 cites W2055043387 @default.
- W2950121979 cites W2068874978 @default.
- W2950121979 cites W2072874994 @default.
- W2950121979 cites W2072970694 @default.
- W2950121979 cites W2074502327 @default.
- W2950121979 cites W2079612411 @default.
- W2950121979 cites W2084350452 @default.
- W2950121979 cites W2089451389 @default.
- W2950121979 cites W2096426946 @default.
- W2950121979 cites W2107768901 @default.
- W2950121979 cites W2112666939 @default.
- W2950121979 cites W2115619791 @default.
- W2950121979 cites W2118751052 @default.
- W2950121979 cites W2123907613 @default.
- W2950121979 cites W2124351063 @default.
- W2950121979 cites W2124525271 @default.
- W2950121979 cites W2124621065 @default.
- W2950121979 cites W2128561900 @default.
- W2950121979 cites W2128880918 @default.
- W2950121979 cites W2130834132 @default.
- W2950121979 cites W2132926880 @default.
- W2950121979 cites W2138176711 @default.
- W2950121979 cites W2142840441 @default.
- W2950121979 cites W2148698435 @default.
- W2950121979 cites W2152443616 @default.
- W2950121979 cites W2157088208 @default.
- W2950121979 cites W2169019412 @default.
- W2950121979 cites W2171468101 @default.
- W2950121979 cites W2172108584 @default.
- W2950121979 cites W2230962545 @default.
- W2950121979 cites W2311203695 @default.
- W2950121979 cites W2400643772 @default.
- W2950121979 cites W2401404581 @default.
- W2950121979 cites W2463726290 @default.
- W2950121979 cites W2599353702 @default.
- W2950121979 cites W2607484736 @default.
- W2950121979 cites W2620918137 @default.
- W2950121979 cites W2751583588 @default.
- W2950121979 cites W2754796163 @default.
- W2950121979 cites W2770974709 @default.
- W2950121979 cites W2813585891 @default.
- W2950121979 cites W2904877754 @default.
- W2950121979 cites W2909399127 @default.
- W2950121979 cites W350234834 @default.
- W2950121979 cites W4229889818 @default.
- W2950121979 cites W4231680290 @default.
- W2950121979 doi "https://doi.org/10.1093/femsec/fiz084" @default.
- W2950121979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31183494" @default.
- W2950121979 hasPublicationYear "2019" @default.
- W2950121979 type Work @default.
- W2950121979 sameAs 2950121979 @default.
- W2950121979 citedByCount "23" @default.
- W2950121979 countsByYear W29501219792019 @default.
- W2950121979 countsByYear W29501219792020 @default.
- W2950121979 countsByYear W29501219792021 @default.
- W2950121979 countsByYear W29501219792022 @default.
- W2950121979 countsByYear W29501219792023 @default.
- W2950121979 crossrefType "journal-article" @default.
- W2950121979 hasAuthorship W2950121979A5017214673 @default.
- W2950121979 hasAuthorship W2950121979A5039418019 @default.
- W2950121979 hasAuthorship W2950121979A5046336339 @default.
- W2950121979 hasAuthorship W2950121979A5087545886 @default.
- W2950121979 hasConcept C104317684 @default.
- W2950121979 hasConcept C18903297 @default.
- W2950121979 hasConcept C193252679 @default.
- W2950121979 hasConcept C2778157034 @default.
- W2950121979 hasConcept C2778867309 @default.