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- W2950152922 abstract "A new method for the synthesis of 2'-O-methyl-2-thiouridine (s2Um) found in thermophilic bacterial tRNA was developed. Structural properties of s2Um and s2Um(p)U were studied by using 1H NMR spectroscopy. A modified nonaribonucleotide (RNA*: 5'-CGUUs2UmUUGC-3') was synthesized to study the base-recognition ability of s2Um in formation of RNA-RNA and RNA DNA duplexes. The UV melting experiments revealed that RNA*-RNA and RNA*-DNA duplexes having an s2U-A base pair are more stable than those having a U-A base pair. On the contrary, the thermal stability of RNA*-RNA and RNA*-DNA duplexes having an s2U-G wobble base pair was much lower than that of the unmodified duplexes having a natural U-G base pair. It is concluded that s2Um has higher selectivity toward A over G than unmodified U." @default.
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- W2950152922 date "2010-05-31" @default.
- W2950152922 modified "2023-09-25" @default.
- W2950152922 title "ChemInform Abstract: Synthesis and Properties of 2′-O-Methyl-2-thiouridine and Oligoribonucleotides Containing 2′-O-Methyl-2-thiouridine." @default.
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- W2950152922 doi "https://doi.org/10.1002/chin.200046228" @default.
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