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- W2950205536 abstract "Three-dimensional single molecule localization microscopy relies on the fitting of the individual molecules with a point spread function (PSF) model. The reconstructed images often show local squeezing or expansion in z. A common cause is depth-induced aberrations in conjunction with an imperfect PSF model calibrated from beads on a coverslip, resulting in a mismatch between measured PSF and real PSF. Here, we developed a strategy for accurate z-localization in which we use the imperfect PSF model for fitting, determine the fitting errors and correct for them in a post-processing step. We present an open-source software tool and a simple experimental calibration procedure that allow retrieving accurate z-positions in any PSF engineering approach or fitting modality, even at large imaging depths." @default.
- W2950205536 created "2019-06-27" @default.
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- W2950205536 date "2019-05-02" @default.
- W2950205536 modified "2023-10-16" @default.
- W2950205536 title "Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization" @default.
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- W2950205536 doi "https://doi.org/10.1364/boe.10.002708" @default.
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