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• W2950243558 abstract "The determination of cell confluency and subculture timing for cell culture consistency is crucial in the field of cell-based research, but there is no universal standard concerning optimal confluence. In this study, gold nanodot arrays on glass substrates were used as culture substrates, and their spectral shifts of localized surface plasmon resonance (LSPR) were employed to monitor cell growth and quantify cell confluency. Experiments including cell counting, metabolic activity, focal adhesion, and cell cycle were also performed to confirm the cell growth monitoring accuracy of the LSPR signals. The LSPR signal exhibited the same trends like the increase of cell numbers and cell metabolic activity and reached the maximum as the cell growth achieved confluency, suggesting its great capability as an effective indicator to predict suitable subculture timing. The proposed sensing approach is a noninterventional, nondestructive, real-time, and useful tool to help biologists quantify the optimal subculture timing, achieve cell culture consistency, and obtain reproducible experimental results efficiently." @default.
• W2950243558 created "2019-06-27" @default.
• W2950243558 creator A5006806681 @default.
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• W2950243558 date "2019-06-18" @default.
• W2950243558 modified "2023-10-16" @default.
• W2950243558 title "Quantifying Cell Confluency by Plasmonic Nanodot Arrays to Achieve Cultivating Consistency" @default.
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• W2950243558 doi "https://doi.org/10.1021/acssensors.9b00524" @default.
• W2950243558 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31251034" @default.
• W2950243558 hasPublicationYear "2019" @default.
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