Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950259567> ?p ?o ?g. }
- W2950259567 endingPage "853" @default.
- W2950259567 startingPage "840" @default.
- W2950259567 abstract "Multiple complexes of 20S proteasomes with accessory factors play an essential role in proteolysis in eukaryotic cells. In this report, several forms of 20S proteasomes from extracts of Spodoptera frugiperda (Sf9) cells were separated using electrophoresis in a native polyacrylamide gel and examined for proteolytic activity in the gel and by Western blotting. Distinct proteasome bands isolated from the gel were subjected to liquid chromatography-tandem mass spectrometry and identified as free core particles (CP) and complexes of CP with one or two dimers of assembly chaperones PAC1-PAC2 and activators PA28γ or PA200. In contrast to the activators PA28γ and PA200 that regulate the access of protein substrates to the internal proteolytic chamber of CP in an ATP-independent manner, the 19S regulatory particle (RP) in 26S proteasomes performs stepwise substrate unfolding and opens the chamber gate in an ATP-dependent manner. Electron microscopic analysis suggested that spontaneous dissociation of RP in isolated 26S proteasomes leaves CPs with different gate sizes related presumably to different stages in the gate opening. The primary structure of 20S proteasome subunits in Sf9 cells was determined by a search of databases and by sequencing. The protein sequences were confirmed by mass spectrometry and verified by 2D gel electrophoresis. The relative rates of sequence divergence in the evolution of 20S proteasome subunits, the assembly chaperones and activators were determined by using bioinformatics. The data confirmed the conservation of regular CP subunits and PA28γ, a more accelerated evolution of PAC2 and PA200, and especially high divergence rates of PAC1." @default.
- W2950259567 created "2019-06-27" @default.
- W2950259567 creator A5003006001 @default.
- W2950259567 creator A5003670699 @default.
- W2950259567 creator A5005351514 @default.
- W2950259567 creator A5013309308 @default.
- W2950259567 creator A5018219936 @default.
- W2950259567 creator A5039177816 @default.
- W2950259567 creator A5043419678 @default.
- W2950259567 creator A5045395710 @default.
- W2950259567 creator A5046318159 @default.
- W2950259567 creator A5061304829 @default.
- W2950259567 creator A5067949064 @default.
- W2950259567 creator A5075469264 @default.
- W2950259567 creator A5080485205 @default.
- W2950259567 creator A5085898312 @default.
- W2950259567 creator A5088613578 @default.
- W2950259567 date "2019-09-01" @default.
- W2950259567 modified "2023-10-14" @default.
- W2950259567 title "Characterization of the 20S proteasome of the lepidopteran, Spodoptera frugiperda" @default.
- W2950259567 cites W1964449627 @default.
- W2950259567 cites W1971841367 @default.
- W2950259567 cites W1988485568 @default.
- W2950259567 cites W1991930087 @default.
- W2950259567 cites W1997054994 @default.
- W2950259567 cites W2000596494 @default.
- W2950259567 cites W2001803273 @default.
- W2950259567 cites W2002772999 @default.
- W2950259567 cites W2009694933 @default.
- W2950259567 cites W2016151751 @default.
- W2950259567 cites W2018755996 @default.
- W2950259567 cites W2024367141 @default.
- W2950259567 cites W2028794261 @default.
- W2950259567 cites W2032194164 @default.
- W2950259567 cites W2033923446 @default.
- W2950259567 cites W2037913550 @default.
- W2950259567 cites W2041714276 @default.
- W2950259567 cites W2041793449 @default.
- W2950259567 cites W2044318442 @default.
- W2950259567 cites W2046164706 @default.
- W2950259567 cites W2059175716 @default.
- W2950259567 cites W2064407050 @default.
- W2950259567 cites W2064746618 @default.
- W2950259567 cites W2066050179 @default.
- W2950259567 cites W2077276173 @default.
- W2950259567 cites W2077322822 @default.
- W2950259567 cites W2083453910 @default.
- W2950259567 cites W2085760247 @default.
- W2950259567 cites W2100455255 @default.
- W2950259567 cites W2105109805 @default.
- W2950259567 cites W2108279225 @default.
- W2950259567 cites W2111647009 @default.
- W2950259567 cites W2119160246 @default.
- W2950259567 cites W2119883540 @default.
- W2950259567 cites W2121673648 @default.
- W2950259567 cites W2124063415 @default.
- W2950259567 cites W2125928564 @default.
- W2950259567 cites W2126467039 @default.
- W2950259567 cites W2140417870 @default.
- W2950259567 cites W2159154670 @default.
- W2950259567 cites W2160128169 @default.
- W2950259567 cites W2170103155 @default.
- W2950259567 cites W2177436571 @default.
- W2950259567 cites W2184475191 @default.
- W2950259567 cites W2277953252 @default.
- W2950259567 cites W2288898583 @default.
- W2950259567 cites W2355140549 @default.
- W2950259567 cites W2484645433 @default.
- W2950259567 cites W2533813884 @default.
- W2950259567 cites W2582325127 @default.
- W2950259567 cites W2593870156 @default.
- W2950259567 cites W2596721500 @default.
- W2950259567 cites W2606700140 @default.
- W2950259567 cites W2730065374 @default.
- W2950259567 cites W2756293157 @default.
- W2950259567 cites W2789554994 @default.
- W2950259567 cites W2798011605 @default.
- W2950259567 cites W2799524357 @default.
- W2950259567 cites W2801061698 @default.
- W2950259567 cites W2802722295 @default.
- W2950259567 cites W2885631076 @default.
- W2950259567 cites W2888498357 @default.
- W2950259567 cites W2890458167 @default.
- W2950259567 cites W2891166165 @default.
- W2950259567 cites W2897820323 @default.
- W2950259567 cites W2898242864 @default.
- W2950259567 cites W2899495934 @default.
- W2950259567 cites W2900290772 @default.
- W2950259567 cites W2911339007 @default.
- W2950259567 cites W2951002538 @default.
- W2950259567 cites W34570582 @default.
- W2950259567 cites W4230719601 @default.
- W2950259567 cites W4247365393 @default.
- W2950259567 doi "https://doi.org/10.1016/j.bbapap.2019.06.010" @default.
- W2950259567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31228587" @default.
- W2950259567 hasPublicationYear "2019" @default.
- W2950259567 type Work @default.
- W2950259567 sameAs 2950259567 @default.