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• W2950343612 abstract "Abstract The advantages of RNA sequencing (RNA-Seq) suggest it will replace microarrays for highly parallel gene expression analysis. For example, in contrast to arrays, RNA-Seq is expected to be able to provide accurate identification and quantification of full-length transcripts. A number of methods have been developed for this purpose, but short error prone reads makes it a difficult problem in practice. It is essential to determine which algorithms perform best, and where and why they fail. However, there is a dearth of independent and unbiased benchmarking studies of these algorithms. Here we take an approach using both simulated and experimental benchmark data to evaluate their accuracy. We conclude that most methods are inaccurate even using idealized data, and that no is method sufficiently accurate once complicating factors such as polymorphisms, intron signal, sequencing error, and multiple splice forms are present. These results point to the pressing need for further algorithm development." @default.
• W2950343612 created "2019-06-27" @default.
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• W2950343612 date "2014-07-14" @default.
• W2950343612 modified "2023-10-14" @default.
• W2950343612 title "Benchmark Analysis of Algorithms for Determining and Quantifying Full-length mRNA Splice Forms from RNA-Seq Data" @default.
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• W2950343612 doi "https://doi.org/10.1101/007088" @default.
• W2950343612 hasPublicationYear "2014" @default.
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