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• W2950355062 abstract "Significance Although it is now possible to visualize single RNA molecules within cells by fluorescence in situ hybridization, the resulting signals are rather faint. Here we describe a method that produces amplified signals from single RNA molecules, while minimizing false-positive signals. Our probes enable detection of sparsely expressed mRNAs, and they are so specific that related mutant and wild-type mRNAs, differing from each other by as little as a single-nucleotide variation, yield distinct in situ signals in 2 distinguishable colors. This technology will aid studies of gene expression phenomena, such as allelic imbalance, and will be useful for the identification of mRNAs harboring somatic mutations in cancer cells, facilitating targeted therapy." @default.
• W2950355062 created "2019-06-27" @default.
• W2950355062 creator A5040039042 @default.
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• W2950355062 date "2019-06-20" @default.
• W2950355062 modified "2023-10-12" @default.
• W2950355062 title "High-fidelity amplified FISH for the detection and allelic discrimination of single mRNA molecules" @default.
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• W2950355062 doi "https://doi.org/10.1073/pnas.1814463116" @default.
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