FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950384946> ?p ?o ?g. }

• W2950384946 endingPage "e0181941" @default.
• W2950384946 startingPage "e0181941" @default.
• W2950384946 abstract "In vitro cell culture is routinely used to grow and supply a sufficiently large number of cells for various types of cell biology experiments. Previous experimental studies report that cell characteristics evolve as the passage number increases, and various cell lines can behave differently at high passage numbers. To provide insight into the putative mechanisms that might give rise to these differences, we perform in silico experiments using a random walk model to mimic the in vitro cell culture process. Our results show that it is possible for the average proliferation rate to either increase or decrease as the passaging process takes place, and this is due to a competition between the initial heterogeneity and the degree to which passaging damages the cells. We also simulate a suite of scratch assays with cells from near-homogeneous and heterogeneous cell lines, at both high and low passage numbers. Although it is common in the literature to report experimental results without disclosing the passage number, our results show that we obtain significantly different closure rates when performing in silico scratch assays using cells with different passage numbers. Therefore, we suggest that the passage number should always be reported to ensure that the experiment is as reproducible as possible. Furthermore, our modelling also suggests some avenues for further experimental examination that could be used to validate or refine our simulation results." @default.
• W2950384946 created "2019-06-27" @default.
• W2950384946 creator A5013897451 @default.
• W2950384946 creator A5018244700 @default.
• W2950384946 creator A5029321517 @default.
• W2950384946 creator A5060813398 @default.
• W2950384946 date "2017-07-27" @default.
• W2950384946 modified "2023-10-05" @default.
• W2950384946 title "A computational modelling framework to quantify the effects of passaging cell lines" @default.
• W2950384946 cites W1964327423 @default.
• W2950384946 cites W1966714020 @default.
• W2950384946 cites W1971402898 @default.
• W2950384946 cites W1975017616 @default.
• W2950384946 cites W1975833234 @default.
• W2950384946 cites W2000832018 @default.
• W2950384946 cites W2011779395 @default.
• W2950384946 cites W2014378137 @default.
• W2950384946 cites W2027542963 @default.
• W2950384946 cites W2047103746 @default.
• W2950384946 cites W2047330128 @default.
• W2950384946 cites W2047611248 @default.
• W2950384946 cites W2051146905 @default.
• W2950384946 cites W2061338580 @default.
• W2950384946 cites W2066003577 @default.
• W2950384946 cites W2067353847 @default.
• W2950384946 cites W2071847433 @default.
• W2950384946 cites W2073399123 @default.
• W2950384946 cites W2100592123 @default.
• W2950384946 cites W2102045126 @default.
• W2950384946 cites W2108080239 @default.
• W2950384946 cites W2111486250 @default.
• W2950384946 cites W2114224807 @default.
• W2950384946 cites W2133811829 @default.
• W2950384946 cites W2148110870 @default.
• W2950384946 cites W2159299299 @default.
• W2950384946 cites W2163404991 @default.
• W2950384946 cites W2163783967 @default.
• W2950384946 cites W2166995984 @default.
• W2950384946 cites W2169160857 @default.
• W2950384946 cites W2171803197 @default.
• W2950384946 cites W2285581648 @default.
• W2950384946 cites W2332464347 @default.
• W2950384946 cites W2412086301 @default.
• W2950384946 cites W2434961035 @default.
• W2950384946 cites W2559649480 @default.
• W2950384946 cites W2605056859 @default.
• W2950384946 cites W2625686163 @default.
• W2950384946 cites W2951467967 @default.
• W2950384946 cites W2951489510 @default.
• W2950384946 cites W2952550896 @default.
• W2950384946 doi "https://doi.org/10.1371/journal.pone.0181941" @default.
• W2950384946 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5531485" @default.
• W2950384946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28750084" @default.
• W2950384946 hasPublicationYear "2017" @default.
• W2950384946 type Work @default.
• W2950384946 sameAs 2950384946 @default.
• W2950384946 citedByCount "14" @default.
• W2950384946 countsByYear W29503849462017 @default.
• W2950384946 countsByYear W29503849462018 @default.
• W2950384946 countsByYear W29503849462019 @default.
• W2950384946 countsByYear W29503849462020 @default.
• W2950384946 countsByYear W29503849462021 @default.
• W2950384946 countsByYear W29503849462022 @default.
• W2950384946 crossrefType "journal-article" @default.
• W2950384946 hasAuthorship W2950384946A5013897451 @default.
• W2950384946 hasAuthorship W2950384946A5018244700 @default.
• W2950384946 hasAuthorship W2950384946A5029321517 @default.
• W2950384946 hasAuthorship W2950384946A5060813398 @default.
• W2950384946 hasBestOaLocation W29503849461 @default.
• W2950384946 hasConcept C104317684 @default.
• W2950384946 hasConcept C1491633281 @default.
• W2950384946 hasConcept C186060115 @default.
• W2950384946 hasConcept C202751555 @default.
• W2950384946 hasConcept C2775905019 @default.
• W2950384946 hasConcept C33618052 @default.
• W2950384946 hasConcept C41008148 @default.
• W2950384946 hasConcept C54355233 @default.
• W2950384946 hasConcept C70721500 @default.
• W2950384946 hasConcept C81885089 @default.
• W2950384946 hasConcept C86803240 @default.
• W2950384946 hasConcept C95444343 @default.
• W2950384946 hasConceptScore W2950384946C104317684 @default.
• W2950384946 hasConceptScore W2950384946C1491633281 @default.
• W2950384946 hasConceptScore W2950384946C186060115 @default.
• W2950384946 hasConceptScore W2950384946C202751555 @default.
• W2950384946 hasConceptScore W2950384946C2775905019 @default.
• W2950384946 hasConceptScore W2950384946C33618052 @default.
• W2950384946 hasConceptScore W2950384946C41008148 @default.
• W2950384946 hasConceptScore W2950384946C54355233 @default.
• W2950384946 hasConceptScore W2950384946C70721500 @default.
• W2950384946 hasConceptScore W2950384946C81885089 @default.
• W2950384946 hasConceptScore W2950384946C86803240 @default.
• W2950384946 hasConceptScore W2950384946C95444343 @default.
• W2950384946 hasFunder F4320334704 @default.
• W2950384946 hasIssue "7" @default.
• W2950384946 hasLocation W29503849461 @default.
• W2950384946 hasLocation W29503849462 @default.
• W2950384946 hasLocation W29503849463 @default.

• next