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- W2950403225 endingPage "1737" @default.
- W2950403225 startingPage "1721" @default.
- W2950403225 abstract "Cleavage factor II (CF II) is a poorly characterized component of the multiprotein complex catalyzing 3′ cleavage and polyadenylation of mammalian mRNA precursors. We have reconstituted CF II as a heterodimer of hPcf11 and hClp1. The heterodimer is active in partially reconstituted cleavage reactions, whereas hClp1 by itself is not. Pcf11 moderately stimulates the RNA 5′ kinase activity of hClp1; the kinase activity is dispensable for RNA cleavage. CF II binds RNA with nanomolar affinity. Binding is mediated mostly by the two zinc fingers in the C-terminal region of hPcf11. RNA is bound without pronounced sequence-specificity, but extended G-rich sequences appear to be preferred. We discuss the possibility that CF II contributes to the recognition of cleavage/polyadenylation substrates through interaction with G-rich far-downstream sequence elements." @default.
- W2950403225 created "2019-06-27" @default.
- W2950403225 creator A5017572254 @default.
- W2950403225 creator A5020104993 @default.
- W2950403225 creator A5034442754 @default.
- W2950403225 creator A5037075303 @default.
- W2950403225 creator A5041558179 @default.
- W2950403225 creator A5043709578 @default.
- W2950403225 creator A5058056443 @default.
- W2950403225 creator A5059822610 @default.
- W2950403225 creator A5076952708 @default.
- W2950403225 creator A5084158849 @default.
- W2950403225 creator A5084226476 @default.
- W2950403225 creator A5090029815 @default.
- W2950403225 date "2018-08-23" @default.
- W2950403225 modified "2023-10-16" @default.
- W2950403225 title "Reconstitution of mammalian cleavage factor II involved in 3′ processing of mRNA precursors" @default.
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