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- W2950407844 abstract "Abstract The synthesis of the novel, fluorescent 3-aminobiphenyl-C-nucleoside M as well as the corresponding building block for oligodeoxynucleotide synthesis is described. M was incorporated into oligodeoxynucleotides via standard phosphoramidite chemistry and the thermal stabilities of duplexes with one and three consecutive M–M, M–P, and M–O pairs, where P denotes an unmodified biphenyl C-nucleoside and O a 3,5-dinitrobiphenyl-C-nucleoside, were determined. It was found that duplexes containing three consecutive M–O pairs were the most stable in the series, notably more stable than a duplex with one additional natural G–C pair instead of the modified residues. Furthermore it was found that the fluorescence of M is efficiently quenched in a duplex when placed opposite to the dinitrophenyl unit O. Thus M and O constitute a novel fluorophore/quencher pair that is orthogonal to natural base pairs in its recognition properties enabling its use as highly specific tags with reporting properties." @default.
- W2950407844 created "2019-06-27" @default.
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- W2950407844 date "2007-06-26" @default.
- W2950407844 modified "2023-09-25" @default.
- W2950407844 title "A Fluorescence-Quencher Pair for DNA Hybridization Studies Based on Hydrophobic Base Surrogates." @default.
- W2950407844 cites W2083157319 @default.
- W2950407844 doi "https://doi.org/10.1002/chin.200726165" @default.
- W2950407844 hasPublicationYear "2007" @default.
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