Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950509320> ?p ?o ?g. }
- W2950509320 abstract "Abstract The genetic determinants of bacterial pathogenicity are highly variable between species and strains. However, a factor that is commonly associated with virulent Gram-negative bacteria, including many Aeromonas spp., is the type 3 secretion system (T3SS), which is used to inject effector proteins into target eukaryotic cells. In this study, we developed a bioinformatics pipeline to identify T3SS effector proteins, applied this approach to the genomes of 105 Aeromonas strains isolated from environmental, mutualistic, or pathogenic contexts and evaluated the cytotoxicity of the identified effectors through their heterologous expression in yeast. The developed pipeline uses a two-step approach, where candidate families are initially selected using HMM profiles with minimal similarity scores against the Virulence Factors DataBase (VFDB), followed by strict comparisons against positive and negative control datasets, greatly reducing the number of false positives. Using our approach, we identified 21 Aeromonas T3SS likely effector groups, of which 8 represented known or characterized effectors, while the remaining 13 had not previously been described in Aeromonas . We experimentally validated our in silico findings by assessing the cytotoxicity of representative effectors in Saccharomyces cerevisiae BY4741, with 15 out of 21 assayed proteins eliciting a cytotoxic effect in yeast. The results of this study demonstrate the utility of our approach, combining a novel in silico search method with in vivo experimental validation, and will be useful in future research aimed at identifying and authenticating bacterial effector proteins from other genera." @default.
- W2950509320 created "2019-06-27" @default.
- W2950509320 creator A5000466819 @default.
- W2950509320 creator A5003531095 @default.
- W2950509320 creator A5012629486 @default.
- W2950509320 creator A5025242251 @default.
- W2950509320 creator A5045259703 @default.
- W2950509320 creator A5060418877 @default.
- W2950509320 date "2019-03-07" @default.
- W2950509320 modified "2023-09-23" @default.
- W2950509320 title "Identification and characterization of putative<i>Aeromonas</i>spp. T3SS effectors" @default.
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