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- W2950585704 abstract "The temporal regulation of protein abundance and post-translational modifications is a key feature of cell division. Recently, we analysed gene expression and protein abundance changes during interphase under minimally perturbed conditions (Ly et al., 2014, 2015). Here, we show that by using specific intracellular immunolabelling protocols, FACS separation of interphase and mitotic cells, including mitotic subphases, can be combined with proteomic analysis by mass spectrometry. Using this PRIMMUS (PRoteomic analysis of Intracellular iMMUnolabelled cell Subsets) approach, we now compare protein abundance and phosphorylation changes in interphase and mitotic fractions from asynchronously growing human cells. We identify a set of 115 phosphorylation sites increased during G2, termed ‘early risers’. This set includes phosphorylation of S738 on TPX2, which we show is important for TPX2 function and mitotic progression. Further, we use PRIMMUS to provide the first a proteome-wide analysis of protein abundance remodeling between prophase, prometaphase and anaphase." @default.
- W2950585704 created "2019-06-27" @default.
- W2950585704 creator A5014112981 @default.
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- W2950585704 date "2017-10-20" @default.
- W2950585704 modified "2023-10-01" @default.
- W2950585704 title "Proteomic analysis of cell cycle progression in asynchronous cultures, including mitotic subphases, using PRIMMUS" @default.
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- W2950585704 doi "https://doi.org/10.7554/elife.27574" @default.
- W2950585704 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5650473" @default.
- W2950585704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29052541" @default.