FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950603264> ?p ?o ?g. }

• W2950603264 endingPage "4460" @default.
• W2950603264 startingPage "4450" @default.
• W2950603264 abstract "DNA alkylation and oxidation are two most common forms of cytotoxic damage with the characteristics of mutagenic and carcinogenic. Human alkyladenine DNA glycosylase (hAAG) is the only glycosylase known to repair a wide variety of alkylative and oxidative DNA lesions. However, few approaches are capable of real-time monitoring hAAG activity. Methods: Herein, we develop a facile fluorescent strategy for homogeneous and sensitive sensing of hAAG activity based on the controllable autocatalytic cleavage-mediated fluorescence recovery. The presence of hAAG enables the cleavage of hairpin probe 1 (HP1) at the damaged 2'-deoxyinosine site by AP endonuclease 1 (APE1), forming a DNA duplex. The trigger 1 built in the resultant DNA duplex may hybridize with hairpin probe 2 (HP2) to induce the T7 exonuclease (T7 exo)-catalyzed recycling cleavage of HP2 (Cycle I) to release trigger 2. The trigger 2 can further hybridize with the signal probe (a fluorophore (FAM) and a quencher (BHQ1) modified at its 5' and 3' ends) to induce the subsequent recycling cleavage of signal probes (Cycle II) to liberate FAM molecules. Through two-recycling autocatalytic cleavage processes, large amounts of fluorophore molecules (i.e., FAM) are liberated from the FAM-BHQ1 fluorescence resonance energy transfer (FRET) pair, leading to the amplified fluorescence recovery. Results: Taking advantage of the high accuracy of in vivo DNA repair mechanism, the high specificity of T7 exo-catalyzed mononucleotides hydrolysis, and the high efficiency of autocatalytic recycling amplification, this strategy exhibits high sensitivity with a detection limit of 4.9 × 10-6 U/μL and a large dynamic range of 4 orders of magnitude from 1 × 10-5 to 0.1 U/μL, and it can further accurately evaluate the enzyme kinetic parameters, screen the potential inhibitors, and even quantify the hAAG activity from 1 cancer cell. Conclusion: The proposed strategy can provide a facile and universal platform for the monitoring of DNA damage-related repair enzymes, holding great potential for DNA repair-related biochemical research, clinical diagnosis, drug discovery, and cancer therapy." @default.
• W2950603264 created "2019-06-27" @default.
• W2950603264 creator A5001568746 @default.
• W2950603264 creator A5053209951 @default.
• W2950603264 creator A5073435344 @default.
• W2950603264 creator A5080412142 @default.
• W2950603264 creator A5086450848 @default.
• W2950603264 creator A5090617065 @default.
• W2950603264 date "2019-01-01" @default.
• W2950603264 modified "2023-09-28" @default.
• W2950603264 title "Controllable Autocatalytic Cleavage-Mediated Fluorescence Recovery for Homogeneous Sensing of Alkyladenine DNA Glycosylase from Human Cancer Cells" @default.
• W2950603264 cites W1509550144 @default.
• W2950603264 cites W1597698279 @default.
• W2950603264 cites W1924651386 @default.
• W2950603264 cites W1963712961 @default.
• W2950603264 cites W1965037168 @default.
• W2950603264 cites W1970900951 @default.
• W2950603264 cites W1973189244 @default.
• W2950603264 cites W1973493626 @default.
• W2950603264 cites W1974471777 @default.
• W2950603264 cites W1974959985 @default.
• W2950603264 cites W1976079395 @default.
• W2950603264 cites W1983367240 @default.
• W2950603264 cites W1991453437 @default.
• W2950603264 cites W2001325691 @default.
• W2950603264 cites W2004173203 @default.
• W2950603264 cites W2009041655 @default.
• W2950603264 cites W2018402427 @default.
• W2950603264 cites W2023963242 @default.
• W2950603264 cites W2034438104 @default.
• W2950603264 cites W2038582507 @default.
• W2950603264 cites W2041804719 @default.
• W2950603264 cites W2058963865 @default.
• W2950603264 cites W2062707431 @default.
• W2950603264 cites W2069705658 @default.
• W2950603264 cites W2071724851 @default.
• W2950603264 cites W2077337585 @default.
• W2950603264 cites W2080490029 @default.
• W2950603264 cites W2086097093 @default.
• W2950603264 cites W2093236036 @default.
• W2950603264 cites W2094144162 @default.
• W2950603264 cites W2107017693 @default.
• W2950603264 cites W2120530347 @default.
• W2950603264 cites W2131494183 @default.
• W2950603264 cites W2139393442 @default.
• W2950603264 cites W2141604349 @default.
• W2950603264 cites W2147696493 @default.
• W2950603264 cites W2164390042 @default.
• W2950603264 cites W2166936616 @default.
• W2950603264 cites W2319343049 @default.
• W2950603264 cites W2328122915 @default.
• W2950603264 cites W2462432763 @default.
• W2950603264 cites W2557858237 @default.
• W2950603264 cites W2595100914 @default.
• W2950603264 cites W2595997208 @default.
• W2950603264 cites W2767681889 @default.
• W2950603264 cites W2898443055 @default.
• W2950603264 cites W2921798683 @default.
• W2950603264 doi "https://doi.org/10.7150/thno.35393" @default.
• W2950603264 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6599653" @default.
• W2950603264 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31285772" @default.
• W2950603264 hasPublicationYear "2019" @default.
• W2950603264 type Work @default.
• W2950603264 sameAs 2950603264 @default.
• W2950603264 citedByCount "13" @default.
• W2950603264 countsByYear W29506032642019 @default.
• W2950603264 countsByYear W29506032642021 @default.
• W2950603264 countsByYear W29506032642022 @default.
• W2950603264 countsByYear W29506032642023 @default.
• W2950603264 crossrefType "journal-article" @default.
• W2950603264 hasAuthorship W2950603264A5001568746 @default.
• W2950603264 hasAuthorship W2950603264A5053209951 @default.
• W2950603264 hasAuthorship W2950603264A5073435344 @default.
• W2950603264 hasAuthorship W2950603264A5080412142 @default.
• W2950603264 hasAuthorship W2950603264A5086450848 @default.
• W2950603264 hasAuthorship W2950603264A5090617065 @default.
• W2950603264 hasBestOaLocation W29506032641 @default.
• W2950603264 hasConcept C121332964 @default.
• W2950603264 hasConcept C12554922 @default.
• W2950603264 hasConcept C143425029 @default.
• W2950603264 hasConcept C151730666 @default.
• W2950603264 hasConcept C175156509 @default.
• W2950603264 hasConcept C185592680 @default.
• W2950603264 hasConcept C192396546 @default.
• W2950603264 hasConcept C2778617687 @default.
• W2950603264 hasConcept C43369102 @default.
• W2950603264 hasConcept C552990157 @default.
• W2950603264 hasConcept C55493867 @default.
• W2950603264 hasConcept C62520636 @default.
• W2950603264 hasConcept C86803240 @default.
• W2950603264 hasConcept C91881484 @default.
• W2950603264 hasConcept C96305047 @default.
• W2950603264 hasConceptScore W2950603264C121332964 @default.
• W2950603264 hasConceptScore W2950603264C12554922 @default.
• W2950603264 hasConceptScore W2950603264C143425029 @default.
• W2950603264 hasConceptScore W2950603264C151730666 @default.
• W2950603264 hasConceptScore W2950603264C175156509 @default.
• W2950603264 hasConceptScore W2950603264C185592680 @default.

• next