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• W2950640147 startingPage "1313" @default.
• W2950640147 abstract "Quantitative imaging methods based on Förster resonance energy transfer (FRET) rely on the determination of an apparent FRET efficiency (Eapp), as well as donor and acceptor concentrations, to uncover the identity and relative abundance of the supramolecular (or quaternary) structures of associating macromolecules. Theoretical work has provided structure-based relationships between Eapp distributions and the quaternary structure models that underlie them. By contrast, the body of work that predicates the signal-based dependence of Eapp on directly measurable quantities (i.e., fluorescence emission of donors and acceptors) relies largely on plausibility arguments, one of which is the seemingly obvious assumption that the fraction of fluorescent molecules in the ground state pretty nearly equals the total concentration of molecules. In this work, we use the kinetic models of fluorescence in the presence and absence of FRET to rigorously derive useful relationships between Eapp and measurable fluorescence signals. Analysis of these relationships reveals a few anticipated results and some unexpected explanations for known experimental FRET puzzles, and it provides theoretical foundations for optimizing measurement strategies." @default.
• W2950640147 created "2019-06-27" @default.
• W2950640147 creator A5044629396 @default.
• W2950640147 date "2019-04-01" @default.
• W2950640147 modified "2023-10-17" @default.
• W2950640147 title "Ab Initio Derivation of the FRET Equations Resolves Old Puzzles and Suggests Measurement Strategies" @default.
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• W2950640147 doi "https://doi.org/10.1016/j.bpj.2019.02.016" @default.
• W2950640147 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6451038" @default.
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