FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950761424> ?p ?o ?g. }

• W2950761424 endingPage "393" @default.
• W2950761424 startingPage "393" @default.
• W2950761424 abstract "Both ricin and R. communis agglutinin (RCA120), belonging to the type II ribosome-inactivating proteins (RIPs-Ⅱ), are derived from the seeds of the castor bean plant. They share very similar amino acid sequences, but ricin is much more toxic than RCA120. It is urgently necessary to distinguish ricin and RCA120 in response to public safety. Currently, mass spectrometric assays are well established for unambiguous identification of ricin by accurate analysis of differentiated amino acid residues after trypsin digestion. However, diagnostic peptides are relatively limited for unambiguous identification of trace ricin, especially in complex matrices. Here, we demonstrate a digestion strategy of multiple proteinases to produce novel peptide markers for unambiguous identification of ricin. Liquid chromatography-high resolution MS (LC-HRMS) was used to verify the resulting peptides, among which only the peptides with uniqueness and good MS response were selected as peptide markers. Seven novel peptide markers were obtained from tandem digestion of trypsin and endoproteinase Glu-C in PBS buffer. From the chymotrypsin digestion under reduction and non-reduction conditions, eight and seven novel peptides were selected respectively. Using pepsin under pH 1~2 and proteinase K digestion, six and five peptides were selected as novel peptide markers. In conclusion, the obtained novel peptides from the established digestion methods can be recommended for the unambiguous identification of ricin during the investigation of illegal use of the toxin." @default.
• W2950761424 created "2019-06-27" @default.
• W2950761424 creator A5001985471 @default.
• W2950761424 creator A5036969586 @default.
• W2950761424 creator A5037906428 @default.
• W2950761424 creator A5044615938 @default.
• W2950761424 creator A5049692788 @default.
• W2950761424 creator A5051852537 @default.
• W2950761424 creator A5060286513 @default.
• W2950761424 creator A5067483347 @default.
• W2950761424 creator A5082580289 @default.
• W2950761424 date "2019-07-05" @default.
• W2950761424 modified "2023-10-16" @default.
• W2950761424 title "LC-HRMS Screening and Identification of Novel Peptide Markers of Ricin Based on Multiple Protease Digestion Strategies" @default.
• W2950761424 cites W1964545646 @default.
• W2950761424 cites W1978276153 @default.
• W2950761424 cites W1979571371 @default.
• W2950761424 cites W1988025447 @default.
• W2950761424 cites W2009109048 @default.
• W2950761424 cites W2017267009 @default.
• W2950761424 cites W2050853916 @default.
• W2950761424 cites W2057281035 @default.
• W2950761424 cites W2084535166 @default.
• W2950761424 cites W2085663493 @default.
• W2950761424 cites W2173402100 @default.
• W2950761424 cites W2173685781 @default.
• W2950761424 cites W2177503722 @default.
• W2950761424 cites W2202199294 @default.
• W2950761424 cites W2329670793 @default.
• W2950761424 cites W2405572757 @default.
• W2950761424 cites W2413758449 @default.
• W2950761424 cites W2419582123 @default.
• W2950761424 cites W2762922052 @default.
• W2950761424 cites W2887446520 @default.
• W2950761424 cites W386311504 @default.
• W2950761424 doi "https://doi.org/10.3390/toxins11070393" @default.
• W2950761424 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6669667" @default.
• W2950761424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31284465" @default.
• W2950761424 hasPublicationYear "2019" @default.
• W2950761424 type Work @default.
• W2950761424 sameAs 2950761424 @default.
• W2950761424 citedByCount "5" @default.
• W2950761424 countsByYear W29507614242019 @default.
• W2950761424 countsByYear W29507614242020 @default.
• W2950761424 countsByYear W29507614242021 @default.
• W2950761424 countsByYear W29507614242022 @default.
• W2950761424 crossrefType "journal-article" @default.
• W2950761424 hasAuthorship W2950761424A5001985471 @default.
• W2950761424 hasAuthorship W2950761424A5036969586 @default.
• W2950761424 hasAuthorship W2950761424A5037906428 @default.
• W2950761424 hasAuthorship W2950761424A5044615938 @default.
• W2950761424 hasAuthorship W2950761424A5049692788 @default.
• W2950761424 hasAuthorship W2950761424A5051852537 @default.
• W2950761424 hasAuthorship W2950761424A5060286513 @default.
• W2950761424 hasAuthorship W2950761424A5067483347 @default.
• W2950761424 hasAuthorship W2950761424A5082580289 @default.
• W2950761424 hasBestOaLocation W29507614241 @default.
• W2950761424 hasConcept C104317684 @default.
• W2950761424 hasConcept C16311235 @default.
• W2950761424 hasConcept C167625842 @default.
• W2950761424 hasConcept C181199279 @default.
• W2950761424 hasConcept C185592680 @default.
• W2950761424 hasConcept C2776714187 @default.
• W2950761424 hasConcept C2777200438 @default.
• W2950761424 hasConcept C2777367657 @default.
• W2950761424 hasConcept C2778471508 @default.
• W2950761424 hasConcept C2779281246 @default.
• W2950761424 hasConcept C2780340462 @default.
• W2950761424 hasConcept C2781307694 @default.
• W2950761424 hasConcept C2781357212 @default.
• W2950761424 hasConcept C43617362 @default.
• W2950761424 hasConcept C515207424 @default.
• W2950761424 hasConcept C55493867 @default.
• W2950761424 hasConceptScore W2950761424C104317684 @default.
• W2950761424 hasConceptScore W2950761424C16311235 @default.
• W2950761424 hasConceptScore W2950761424C167625842 @default.
• W2950761424 hasConceptScore W2950761424C181199279 @default.
• W2950761424 hasConceptScore W2950761424C185592680 @default.
• W2950761424 hasConceptScore W2950761424C2776714187 @default.
• W2950761424 hasConceptScore W2950761424C2777200438 @default.
• W2950761424 hasConceptScore W2950761424C2777367657 @default.
• W2950761424 hasConceptScore W2950761424C2778471508 @default.
• W2950761424 hasConceptScore W2950761424C2779281246 @default.
• W2950761424 hasConceptScore W2950761424C2780340462 @default.
• W2950761424 hasConceptScore W2950761424C2781307694 @default.
• W2950761424 hasConceptScore W2950761424C2781357212 @default.
• W2950761424 hasConceptScore W2950761424C43617362 @default.
• W2950761424 hasConceptScore W2950761424C515207424 @default.
• W2950761424 hasConceptScore W2950761424C55493867 @default.
• W2950761424 hasIssue "7" @default.
• W2950761424 hasLocation W29507614241 @default.
• W2950761424 hasLocation W29507614242 @default.
• W2950761424 hasLocation W29507614243 @default.
• W2950761424 hasLocation W29507614244 @default.
• W2950761424 hasLocation W29507614245 @default.
• W2950761424 hasLocation W29507614246 @default.
• W2950761424 hasOpenAccess W2950761424 @default.
• W2950761424 hasPrimaryLocation W29507614241 @default.

• next