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- W2950840691 endingPage "1206" @default.
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- W2950840691 abstract "To evaluate efficacy of Saccharomyces cerevisiae fermentate prebiotic (EH) in protection of intestinal barrier integrity in rats during heat stress, to analyze the impact of heat stress and preventive treatment with EH on the structure of the gut microbiota.Two groups of rats were treated orally with EH or phosphate-buffered saline for 14 days. On day 15, half of the rats in each group were exposed to heat stress conditions, while control animals were kept at room temperature. Histological and Western blot analyses of the intestine, culture-based microbiological analysis and high-throughput 16S rRNA sequencing for the gut microbiota were performed for each rat. Exposure of animals to heat stress conditions resulted in inhibition of tight junction (TJ) proteins expression, decrease of Paneth and goblet cells, decrease of beneficial and increase of pathogenic bacteria. Oral treatment of rats with EH before stress significantly prevents these adverse effects by elevation of the gut beneficial bacteria, particularly butyrate-producing bacteria.Essential effect of EH in protection of intestinal barrier integrity during heat stress is connected with beneficial modulation of the gut microbiota.Our results will contribute to the development of new approaches to prevention of heat stress-related complications." @default.
- W2950840691 created "2019-06-27" @default.
- W2950840691 creator A5014155120 @default.
- W2950840691 creator A5031823632 @default.
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- W2950840691 creator A5074152530 @default.
- W2950840691 creator A5077714054 @default.
- W2950840691 creator A5085059141 @default.
- W2950840691 date "2019-07-09" @default.
- W2950840691 modified "2023-10-14" @default.
- W2950840691 title "Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats" @default.
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