FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950844507> ?p ?o ?g. }

• W2950844507 endingPage "16" @default.
• W2950844507 startingPage "1" @default.
• W2950844507 abstract "Autophagy is a degradative process in which cellular organelles and proteins are recycled to restore homeostasis and cellular metabolism. Autophagy can be either a prosurvival or a prodeath process and remains one of the most fundamental processes for cell vitality. Thus autophagy modulation is an important approach for reinforcement anticancer therapeutics. Earlier we have demonstrated that recombinant analog of human milk protein lactaptin (RL2) induced apoptosis of various cultured cancer cells and activated lipidation of microtubule-associated protein 1 light chain 3 (LC3). In this study we investigated whether autophagy inhibitors—chloroquine (CQ), Ku55933 (Ku), and 3-methyladenine (3MA)—or inducer—rapamycin (Rap)—can enhance cytotoxic activity of lactaptin analog in cancer cells and its anticancer activity in the mice model. Western Blot analysis revealed that RL2 induced short-term autophagy in MDA-MB-231 and MCF-7 cells at early stages of incubation and that these data were confirmed by the transmission electron microscopy of autophagosome/autophagolysosome formation. RL2 stimulates reactive oxygen species (ROS) production, autophagosomes accumulation, upregulation of ATG5 with processing of LC3I to LC3II, and downregulation of p62/sequestosome 1 (p62). We have shown that autophagy modulators, CQ, Ku, and Rap, synergistically increased cytotoxicity of RL2, and RL2 with CQ induced autophagic cell death. In addition, CQ, Ku, and Rap in combination with RL2 decreased activity of lysosomal protease Cathepsin D. More importantly, combining RL2 with CQ, we improved antitumor effect in mice. Detected synergistic cytotoxic effects of both types of autophagy regulators, inhibitors, and inducers with RL2 against cancer cells allow us to believe that these combinations can be a basis for the new anticancer approach. Finally, we suppose that CQ and Rap promoting of short-term RL2-induced autophagy interlinks with final autophagic cell death." @default.
• W2950844507 created "2019-06-27" @default.
• W2950844507 creator A5003498731 @default.
• W2950844507 creator A5007850840 @default.
• W2950844507 creator A5009511937 @default.
• W2950844507 creator A5026381492 @default.
• W2950844507 creator A5029566293 @default.
• W2950844507 creator A5037011954 @default.
• W2950844507 creator A5041343915 @default.
• W2950844507 creator A5051450762 @default.
• W2950844507 creator A5054417011 @default.
• W2950844507 creator A5075940354 @default.
• W2950844507 creator A5085616388 @default.
• W2950844507 creator A5088248360 @default.
• W2950844507 creator A5076499148 @default.
• W2950844507 date "2019-06-17" @default.
• W2950844507 modified "2023-10-01" @default.
• W2950844507 title "Cytotoxic and Antitumor Activity of Lactaptin in Combination with Autophagy Inducers and Inhibitors" @default.
• W2950844507 cites W1963521777 @default.
• W2950844507 cites W1968825280 @default.
• W2950844507 cites W1978621808 @default.
• W2950844507 cites W1990328562 @default.
• W2950844507 cites W1992617406 @default.
• W2950844507 cites W1999998145 @default.
• W2950844507 cites W2002408632 @default.
• W2950844507 cites W2003887174 @default.
• W2950844507 cites W2014316690 @default.
• W2950844507 cites W2025665129 @default.
• W2950844507 cites W2043028930 @default.
• W2950844507 cites W2053038488 @default.
• W2950844507 cites W2059107485 @default.
• W2950844507 cites W2059773837 @default.
• W2950844507 cites W2072301202 @default.
• W2950844507 cites W2074350582 @default.
• W2950844507 cites W2087347365 @default.
• W2950844507 cites W2087807630 @default.
• W2950844507 cites W2091597079 @default.
• W2950844507 cites W2097204034 @default.
• W2950844507 cites W2101606711 @default.
• W2950844507 cites W2115772647 @default.
• W2950844507 cites W2133415244 @default.
• W2950844507 cites W2135574023 @default.
• W2950844507 cites W2151422820 @default.
• W2950844507 cites W2151485523 @default.
• W2950844507 cites W2163970515 @default.
• W2950844507 cites W2328267773 @default.
• W2950844507 cites W2345873911 @default.
• W2950844507 cites W2397181796 @default.
• W2950844507 cites W2511991663 @default.
• W2950844507 cites W2519786994 @default.
• W2950844507 cites W2570859884 @default.
• W2950844507 cites W2592067246 @default.
• W2950844507 cites W2598101424 @default.
• W2950844507 cites W2728497530 @default.
• W2950844507 cites W2751980546 @default.
• W2950844507 cites W2769842768 @default.
• W2950844507 cites W2792410331 @default.
• W2950844507 cites W2797244031 @default.
• W2950844507 cites W2808021744 @default.
• W2950844507 cites W2889331509 @default.
• W2950844507 cites W2890999095 @default.
• W2950844507 cites W2899871244 @default.
• W2950844507 cites W2913554923 @default.
• W2950844507 cites W3187318779 @default.
• W2950844507 cites W4242113921 @default.
• W2950844507 doi "https://doi.org/10.1155/2019/4087160" @default.
• W2950844507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6601476" @default.
• W2950844507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31317028" @default.
• W2950844507 hasPublicationYear "2019" @default.
• W2950844507 type Work @default.
• W2950844507 sameAs 2950844507 @default.
• W2950844507 citedByCount "7" @default.
• W2950844507 countsByYear W29508445072020 @default.
• W2950844507 countsByYear W29508445072021 @default.
• W2950844507 countsByYear W29508445072023 @default.
• W2950844507 crossrefType "journal-article" @default.
• W2950844507 hasAuthorship W2950844507A5003498731 @default.
• W2950844507 hasAuthorship W2950844507A5007850840 @default.
• W2950844507 hasAuthorship W2950844507A5009511937 @default.
• W2950844507 hasAuthorship W2950844507A5026381492 @default.
• W2950844507 hasAuthorship W2950844507A5029566293 @default.
• W2950844507 hasAuthorship W2950844507A5037011954 @default.
• W2950844507 hasAuthorship W2950844507A5041343915 @default.
• W2950844507 hasAuthorship W2950844507A5051450762 @default.
• W2950844507 hasAuthorship W2950844507A5054417011 @default.
• W2950844507 hasAuthorship W2950844507A5075940354 @default.
• W2950844507 hasAuthorship W2950844507A5076499148 @default.
• W2950844507 hasAuthorship W2950844507A5085616388 @default.
• W2950844507 hasAuthorship W2950844507A5088248360 @default.
• W2950844507 hasBestOaLocation W29508445071 @default.
• W2950844507 hasConcept C104317684 @default.
• W2950844507 hasConcept C109316439 @default.
• W2950844507 hasConcept C127561419 @default.
• W2950844507 hasConcept C154317977 @default.
• W2950844507 hasConcept C185592680 @default.
• W2950844507 hasConcept C190283241 @default.
• W2950844507 hasConcept C202751555 @default.
• W2950844507 hasConcept C203522944 @default.

• next