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• W2950855923 abstract "Abstract The recently developed droplet-based single cell transcriptome sequencing (scRNA-seq) technology makes it feasible to perform a population-scale scRNA-seq study, in which the transcriptome is measured for tens of thousands of single cells from multiple individuals. Despite the advances of many clustering methods, there are few tailored methods for population-scale scRNA-seq studies. Here, we have developed a B Ayesiany M ixture M odel for S ingle C ell sequencing (BAMM-SC) method to cluster scRNA-seq data from multiple individuals simultaneously. Specifically, BAMM-SC takes raw data as input and can account for data heterogeneity and batch effect among multiple individuals in a unified Bayesian hierarchical model framework. Results from extensive simulations and application of BAMM-SC to in-house scRNA-seq datasets using blood, lung and skin cells from humans or mice demonstrated that BAMM-SC outperformed existing clustering methods with improved clustering accuracy and reduced impact from batch effects. BAMM-SC has been implemented in a user-friendly R package with a detailed tutorial available on www.pitt.edu/~Cwec47/singlecell.html ." @default.
• W2950855923 created "2019-06-27" @default.
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• W2950855923 date "2018-08-16" @default.
• W2950855923 modified "2023-10-02" @default.
• W2950855923 title "BAMM-SC: A Bayesian mixture model for clustering droplet-based single cell transcriptomic data from population studies" @default.
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• W2950855923 doi "https://doi.org/10.1101/392662" @default.
• W2950855923 hasPublicationYear "2018" @default.
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