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• W2950890178 abstract "The bio-economy relies on microbial strains optimized for efficient large scale production of chemicals and fuels from inexpensive and renewable feedstocks under industrial conditions. The reduced one carbon compound methanol, whose production does not involve carbohydrates needed for the feed and food sector, can be used as sole carbon and energy source by methylotrophic bacteria like Methylobacterium extorquens AM1. This strain has already been engineered to produce various commodity and high value chemicals from methanol. The toxic effect of methanol limits its concentration as feedstock to 1% v/v. We obtained M. extorquens chassis strains tolerant to high methanol via adaptive directed evolution using the GM3 technology of automated continuous culture. Turbidostat and conditional medium swap regimes were employed for the parallel evolution of the recently characterized strain TK 0001 and the reference strain AM1 and enabled the isolation of derivatives of both strains capable of stable growth with 10% methanol. The isolates produced more biomass at 1% methanol than the ancestor strains. Genome sequencing identified the gene metY coding for an O-acetyl-L-homoserine-sulfhydrylase as common target of mutation. We showed that the wildtype enzyme uses methanol as substrate at elevated concentrations. This side reaction would produce methoxine, a toxic homolog of methionine incorporated in polypeptides during translation. All mutated metY alleles isolated from the evolved populations coded for inactive enzymes, designating O-acetyl-L-homoserine sulfhydrylase as a major vector of methanol toxicity. A whole cell transcriptomic analysis revealed that genes coding for chaperones and proteases were upregulated in the evolved cells as compared with the wildtype, suggesting that the cells had to cope with aberrant proteins formed during the adaptation to increasing methanol exposure. In addition, the expression of ribosomal proteins and enzymes related to energy production from methanol like formate dehydrogenases and ATP synthases was boosted in the evolved cells upon a short-term methanol stress. D-lactate production from methanol by adapted cells overexpressing the native D-lactate dehydrogenase was quantified. A significant higher lactate yield was obtained compared with control cells, indicating an enhanced capacity of the cells resistant to high methanol to assimilate this one carbon feedstock more efficiently." @default.
• W2950890178 created "2019-06-27" @default.
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• W2950890178 date "2019-06-20" @default.
• W2950890178 modified "2023-10-01" @default.
• W2950890178 title "Continuous Culture Adaptation of Methylobacterium extorquens AM1 and TK 0001 to Very High Methanol Concentrations" @default.
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• W2950890178 cites W1970051672 @default.
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• W2950890178 cites W1970912575 @default.
• W2950890178 cites W1972412013 @default.
• W2950890178 cites W1992094275 @default.
• W2950890178 cites W2001649514 @default.
• W2950890178 cites W2002997817 @default.
• W2950890178 cites W2004791437 @default.
• W2950890178 cites W2008127077 @default.
• W2950890178 cites W2010451964 @default.
• W2950890178 cites W2014723793 @default.
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• W2950890178 cites W2027898383 @default.
• W2950890178 cites W2033511085 @default.
• W2950890178 cites W2035512890 @default.
• W2950890178 cites W2039510597 @default.
• W2950890178 cites W2043105926 @default.
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• W2950890178 cites W2069425295 @default.
• W2950890178 cites W2079656901 @default.
• W2950890178 cites W2082140226 @default.
• W2950890178 cites W2084333783 @default.
• W2950890178 cites W2086655471 @default.
• W2950890178 cites W2087064593 @default.
• W2950890178 cites W2091697983 @default.
• W2950890178 cites W2092668948 @default.
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• W2950890178 cites W2105979475 @default.
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• W2950890178 cites W2119964499 @default.
• W2950890178 cites W2124078475 @default.
• W2950890178 cites W2142619120 @default.
• W2950890178 cites W2148590972 @default.
• W2950890178 cites W2152239989 @default.
• W2950890178 cites W2155575080 @default.
• W2950890178 cites W2157312398 @default.
• W2950890178 cites W2160835140 @default.
• W2950890178 cites W2162895373 @default.
• W2950890178 cites W2165391691 @default.
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• W2950890178 cites W2303015818 @default.
• W2950890178 cites W2323407309 @default.
• W2950890178 cites W2330621114 @default.
• W2950890178 cites W2399010589 @default.
• W2950890178 cites W2414124995 @default.
• W2950890178 cites W2531330468 @default.
• W2950890178 cites W2531471130 @default.
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• W2950890178 doi "https://doi.org/10.3389/fmicb.2019.01313" @default.
• W2950890178 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6595629" @default.
• W2950890178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31281294" @default.
• W2950890178 hasPublicationYear "2019" @default.
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