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- W2950905288 abstract "SUMMARY Growing evidence implicates the importance of glia, particularly astrocytes, in neurological and psychiatric diseases. Here, we describe a rapid and robust method for the differentiation of highly pure populations of replicative astrocytes from human induced pluripotent stem cells (hiPSCs), via a neural progenitor cell (NPC) intermediate. Using this method, we generated hiPSC-derived astrocyte populations (hiPSC-astrocytes) from 42 NPC lines (derived from 30 individuals) with an average of ∼90% S100β-positive cells. Transcriptomic analysis demonstrated that the hiPSC-astrocytes are highly similar to primary human fetal astrocytes and characteristic of a non-reactive state. hiPSC-astrocytes respond to inflammatory stimulants, display phagocytic capacity and enhance microglial phagocytosis. hiPSC-astrocytes also possess spontaneous calcium transient activity. Our novel protocol is a reproducible, straightforward (single media) and rapid (<30 days) method to generate homogenous populations of hiPSC-astrocytes that can be used for neuron-astrocyte and microglia-astrocyte co-cultures for the study of neuropsychiatric disorders. ABBREVIATIONS hiPSC human induced pluripotent stem cell NPC neural progenitor cell" @default.
- W2950905288 created "2019-06-27" @default.
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- W2950905288 date "2017-05-03" @default.
- W2950905288 modified "2023-09-27" @default.
- W2950905288 title "An efficient platform for astrocyte differentiation from human induced pluripotent stem cells" @default.
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- W2950905288 doi "https://doi.org/10.1101/133496" @default.
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