Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950921485> ?p ?o ?g. }
- W2950921485 abstract "Abstract The outcome of a host-pathogen interaction is determined by the conditions of the host, the pathogen, and the environment. Although numerous proteomic studies of in vitro-grown microbial pathogens have been performed, in vivo proteomic approaches are still rare. In addition, increasing evidence supports that in vitro studies inadequately reflect in vivo conditions. Choosing the proper host is essential to detect the expression of proteins from the pathogen in vivo. Numerous studies have demonstrated the suitability of zebrafish ( Danio rerio ) embryos as a model to in vivo studies of Pseudomonas aeruginosa infection. In most zebrafish-pathogen studies, infection is achieved by microinjection of bacteria into the larvae. However, few reports using static immersion of bacterial pathogens have been published. In this study we infected 3 days post-fertilization (DPF) zebrafish larvae with P. aeruginosa PAO1 by immersion and injection and tracked the in vivo immune response by the zebrafish. Additionally, by using non-isotopic (Q-exactive) metaproteomics we simultaneously evaluated the proteomic response of the pathogen ( P. aeruginosa PAO1) and the host (zebrafish). We found some zebrafish metabolic pathways, such as hypoxia response via HIF activation pathway, exclusively enriched in the larvae exposed by static immersion. In contrast, we found that inflammation mediated by chemokine and cytokine signaling pathways was exclusively enriched in the larvae exposed by injection, while the integrin signaling pathway and angiogenesis were solely enriched in the larvae exposed by immersion. We also found important virulence factors from P. aeruginosa that were enriched only after exposure by injection, such as the Type-III secretion system and flagella-associated proteins. On the other hand, P. aeruginosa proteins involved in processes like biofilm formation, cellular responses to antibiotic and starvation were enriched exclusively after an exposure by immersion. We demonstrated the suitability of zebrafish embryos as a model for in vivo host-pathogen based proteomic studies in P. aeruginosa . Our global proteomic profiling identifies novel molecular signatures that give systematic insight into zebrafish -Pseudomonas interaction." @default.
- W2950921485 created "2019-06-27" @default.
- W2950921485 creator A5021195881 @default.
- W2950921485 creator A5022891782 @default.
- W2950921485 creator A5048805238 @default.
- W2950921485 creator A5063571990 @default.
- W2950921485 creator A5087346280 @default.
- W2950921485 date "2017-05-21" @default.
- W2950921485 modified "2023-10-18" @default.
- W2950921485 title "<i>In vivo</i>host-pathogen interaction as revealed by global proteomic profiling of zebrafish larvae" @default.
- W2950921485 cites W1828093076 @default.
- W2950921485 cites W1885759691 @default.
- W2950921485 cites W1925832310 @default.
- W2950921485 cites W1965917470 @default.
- W2950921485 cites W1975510036 @default.
- W2950921485 cites W1978334623 @default.
- W2950921485 cites W1984049034 @default.
- W2950921485 cites W1986656413 @default.
- W2950921485 cites W1990484548 @default.
- W2950921485 cites W1992332107 @default.
- W2950921485 cites W1993691476 @default.
- W2950921485 cites W1996577630 @default.
- W2950921485 cites W2006342320 @default.
- W2950921485 cites W2010041610 @default.
- W2950921485 cites W2014553102 @default.
- W2950921485 cites W2015967067 @default.
- W2950921485 cites W2017762021 @default.
- W2950921485 cites W2020179710 @default.
- W2950921485 cites W2021185780 @default.
- W2950921485 cites W2022472106 @default.
- W2950921485 cites W2022770755 @default.
- W2950921485 cites W2037199116 @default.
- W2950921485 cites W2039830769 @default.
- W2950921485 cites W2047275456 @default.
- W2950921485 cites W2048630989 @default.
- W2950921485 cites W2051368254 @default.
- W2950921485 cites W2052388444 @default.
- W2950921485 cites W2068016108 @default.
- W2950921485 cites W2070214421 @default.
- W2950921485 cites W2077024843 @default.
- W2950921485 cites W2077665732 @default.
- W2950921485 cites W2081465448 @default.
- W2950921485 cites W2104817697 @default.
- W2950921485 cites W2114794862 @default.
- W2950921485 cites W2116063068 @default.
- W2950921485 cites W2119175743 @default.
- W2950921485 cites W2123679840 @default.
- W2950921485 cites W2129514750 @default.
- W2950921485 cites W2130522035 @default.
- W2950921485 cites W2130706354 @default.
- W2950921485 cites W2145943628 @default.
- W2950921485 cites W2148993454 @default.
- W2950921485 cites W2151098931 @default.
- W2950921485 cites W2163803918 @default.
- W2950921485 cites W2166252406 @default.
- W2950921485 cites W2184156621 @default.
- W2950921485 cites W2515712022 @default.
- W2950921485 cites W2739999456 @default.
- W2950921485 doi "https://doi.org/10.1101/140509" @default.
- W2950921485 hasPublicationYear "2017" @default.
- W2950921485 type Work @default.
- W2950921485 sameAs 2950921485 @default.
- W2950921485 citedByCount "0" @default.
- W2950921485 crossrefType "posted-content" @default.
- W2950921485 hasAuthorship W2950921485A5021195881 @default.
- W2950921485 hasAuthorship W2950921485A5022891782 @default.
- W2950921485 hasAuthorship W2950921485A5048805238 @default.
- W2950921485 hasAuthorship W2950921485A5063571990 @default.
- W2950921485 hasAuthorship W2950921485A5087346280 @default.
- W2950921485 hasBestOaLocation W29509214851 @default.
- W2950921485 hasConcept C104317684 @default.
- W2950921485 hasConcept C13373296 @default.
- W2950921485 hasConcept C203014093 @default.
- W2950921485 hasConcept C207001950 @default.
- W2950921485 hasConcept C2776460866 @default.
- W2950921485 hasConcept C2776878037 @default.
- W2950921485 hasConcept C2777637488 @default.
- W2950921485 hasConcept C2778375072 @default.
- W2950921485 hasConcept C2780384893 @default.
- W2950921485 hasConcept C523546767 @default.
- W2950921485 hasConcept C54355233 @default.
- W2950921485 hasConcept C60987743 @default.
- W2950921485 hasConcept C86803240 @default.
- W2950921485 hasConcept C8891405 @default.
- W2950921485 hasConcept C89423630 @default.
- W2950921485 hasConcept C95444343 @default.
- W2950921485 hasConceptScore W2950921485C104317684 @default.
- W2950921485 hasConceptScore W2950921485C13373296 @default.
- W2950921485 hasConceptScore W2950921485C203014093 @default.
- W2950921485 hasConceptScore W2950921485C207001950 @default.
- W2950921485 hasConceptScore W2950921485C2776460866 @default.
- W2950921485 hasConceptScore W2950921485C2776878037 @default.
- W2950921485 hasConceptScore W2950921485C2777637488 @default.
- W2950921485 hasConceptScore W2950921485C2778375072 @default.
- W2950921485 hasConceptScore W2950921485C2780384893 @default.
- W2950921485 hasConceptScore W2950921485C523546767 @default.
- W2950921485 hasConceptScore W2950921485C54355233 @default.
- W2950921485 hasConceptScore W2950921485C60987743 @default.
- W2950921485 hasConceptScore W2950921485C86803240 @default.
- W2950921485 hasConceptScore W2950921485C8891405 @default.