Matches in SemOpenAlex for { <https://semopenalex.org/work/W2950931723> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2950931723 endingPage "226" @default.
- W2950931723 startingPage "221" @default.
- W2950931723 abstract "Quantitative analysis of next-generation sequencing (NGS) data requires discriminating duplicate reads generated by PCR from identical molecules that are of unique origin. Typically, PCR duplicates are identified as sequence reads that align to the same genomic coordinates using reference-based alignment. However, identical molecules can be independently generated during library preparation. Misidentification of these molecules as PCR duplicates can introduce unforeseen biases during analyses. Here, we developed a cost-effective sequencing adapter design by modifying Illumina TruSeq adapters to incorporate a unique molecular identifier (UMI) while maintaining the capacity to undertake multiplexed, single-index sequencing. Incorporation of UMIs into TruSeq adapters (TrUMIseq adapters) enables identification of bona fide PCR duplicates as identically mapped reads with identical UMIs. Using TrUMIseq adapters, we show that accurate removal of PCR duplicates results in improved accuracy of both allele frequency (AF) estimation in heterogeneous populations using DNA sequencing and gene expression quantification using RNA-Seq." @default.
- W2950931723 created "2019-06-27" @default.
- W2950931723 creator A5004372835 @default.
- W2950931723 creator A5076191388 @default.
- W2950931723 date "2017-11-01" @default.
- W2950931723 modified "2023-10-14" @default.
- W2950931723 title "Incorporation of unique molecular identifiers in TruSeq adapters improves the accuracy of quantitative sequencing" @default.
- W2950931723 cites W1988391018 @default.
- W2950931723 cites W1999518415 @default.
- W2950931723 cites W2011645981 @default.
- W2950931723 cites W2019955264 @default.
- W2950931723 cites W2028319246 @default.
- W2950931723 cites W2041677964 @default.
- W2950931723 cites W2065324308 @default.
- W2950931723 cites W2084942072 @default.
- W2950931723 cites W2097895258 @default.
- W2950931723 cites W2119278037 @default.
- W2950931723 cites W2160444236 @default.
- W2950931723 cites W2747545374 @default.
- W2950931723 cites W2951890476 @default.
- W2950931723 cites W2951951814 @default.
- W2950931723 doi "https://doi.org/10.2144/000114608" @default.
- W2950931723 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7359820" @default.
- W2950931723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29185922" @default.
- W2950931723 hasPublicationYear "2017" @default.
- W2950931723 type Work @default.
- W2950931723 sameAs 2950931723 @default.
- W2950931723 citedByCount "35" @default.
- W2950931723 countsByYear W29509317232018 @default.
- W2950931723 countsByYear W29509317232019 @default.
- W2950931723 countsByYear W29509317232020 @default.
- W2950931723 countsByYear W29509317232021 @default.
- W2950931723 countsByYear W29509317232022 @default.
- W2950931723 countsByYear W29509317232023 @default.
- W2950931723 crossrefType "journal-article" @default.
- W2950931723 hasAuthorship W2950931723A5004372835 @default.
- W2950931723 hasAuthorship W2950931723A5076191388 @default.
- W2950931723 hasBestOaLocation W29509317231 @default.
- W2950931723 hasConcept C111919701 @default.
- W2950931723 hasConcept C177284502 @default.
- W2950931723 hasConcept C192953774 @default.
- W2950931723 hasConcept C41008148 @default.
- W2950931723 hasConcept C51679486 @default.
- W2950931723 hasConcept C54355233 @default.
- W2950931723 hasConcept C552990157 @default.
- W2950931723 hasConcept C70721500 @default.
- W2950931723 hasConcept C86803240 @default.
- W2950931723 hasConceptScore W2950931723C111919701 @default.
- W2950931723 hasConceptScore W2950931723C177284502 @default.
- W2950931723 hasConceptScore W2950931723C192953774 @default.
- W2950931723 hasConceptScore W2950931723C41008148 @default.
- W2950931723 hasConceptScore W2950931723C51679486 @default.
- W2950931723 hasConceptScore W2950931723C54355233 @default.
- W2950931723 hasConceptScore W2950931723C552990157 @default.
- W2950931723 hasConceptScore W2950931723C70721500 @default.
- W2950931723 hasConceptScore W2950931723C86803240 @default.
- W2950931723 hasIssue "5" @default.
- W2950931723 hasLocation W29509317231 @default.
- W2950931723 hasLocation W29509317232 @default.
- W2950931723 hasLocation W29509317233 @default.
- W2950931723 hasLocation W29509317234 @default.
- W2950931723 hasLocation W29509317235 @default.
- W2950931723 hasLocation W29509317236 @default.
- W2950931723 hasOpenAccess W2950931723 @default.
- W2950931723 hasPrimaryLocation W29509317231 @default.
- W2950931723 hasRelatedWork W2105035271 @default.
- W2950931723 hasRelatedWork W2107233609 @default.
- W2950931723 hasRelatedWork W2485605994 @default.
- W2950931723 hasRelatedWork W2981692913 @default.
- W2950931723 hasRelatedWork W3044188621 @default.
- W2950931723 hasRelatedWork W3163755236 @default.
- W2950931723 hasRelatedWork W3184035966 @default.
- W2950931723 hasRelatedWork W4306381730 @default.
- W2950931723 hasRelatedWork W4385571108 @default.
- W2950931723 hasRelatedWork W4229060448 @default.
- W2950931723 hasVolume "63" @default.
- W2950931723 isParatext "false" @default.
- W2950931723 isRetracted "false" @default.
- W2950931723 magId "2950931723" @default.
- W2950931723 workType "article" @default.