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• W2950973927 abstract "The clustered regularly interspaced short palindromic repeat (CRISPR) system is widely used for various biological applications, including genome editing. We developed engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR to isolate target genomic regions from cells for their biochemical characterization. In this study, we developed 'in vitro enChIP' using recombinant CRISPR ribonucleoproteins (RNPs) to isolate target genomic regions. in vitro enChIP has the great advantage over conventional enChIP of not requiring expression of CRISPR complexes in cells. We first demonstrate that in vitro enChIP using recombinant CRISPR RNPs can be used to isolate target DNA from mixtures of purified DNA in a sequence-specific manner. In addition, we show that this technology can be employed to efficiently isolate target genomic regions, while retaining their intracellular molecular interactions, with negligible contamination from irrelevant genomic regions. Thus, in vitro enChIP technology is of potential use for sequence-specific isolation of DNA, as well as for identification of molecules interacting with genomic regions of interest in vivo in combination with downstream analysis." @default.
• W2950973927 created "2019-06-27" @default.
• W2950973927 creator A5052933407 @default.
• W2950973927 creator A5075111839 @default.
• W2950973927 creator A5080519234 @default.
• W2950973927 date "2015-11-30" @default.
• W2950973927 modified "2023-09-23" @default.
• W2950973927 title "Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins" @default.
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• W2950973927 doi "https://doi.org/10.1101/033241" @default.
• W2950973927 hasPublicationYear "2015" @default.
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