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• W2950996676 abstract "Single-wavelength fluorescent reporters allow visualization of specific neurotransmitters with high spatial and temporal resolution. We report variants of intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) that are functionally brighter; detect submicromolar to millimolar amounts of glutamate; and have blue, cyan, green, or yellow emission profiles. These variants could be imaged in vivo in cases where original iGluSnFR was too dim, resolved glutamate transients in dendritic spines and axonal boutons, and allowed imaging at kilohertz rates. Variants of the genetically encoded sensor iGluSnFR extend the range of conditions under which glutamate neurotransmission can be visualized. In addition, chromatic variants of iGluSnFR improve compatibility with various illumination schemes." @default.
• W2950996676 created "2019-06-27" @default.
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• W2950996676 date "2018-10-30" @default.
• W2950996676 modified "2023-10-18" @default.
• W2950996676 title "Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR" @default.
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• W2950996676 doi "https://doi.org/10.1038/s41592-018-0171-3" @default.
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