Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951004074> ?p ?o ?g. }
- W2951004074 abstract "ABSTRACT Brucella spp are intracellular pathogenic bacteria remarkable in their ability to escape immune surveillance and therefore inflict a state of chronic disease within the host. To enable further immune response studies, Brucella were engineered to express the well characterized chicken ovalbumin (OVA). Surprisingly, we found that CD8 T cells bearing T cell receptors (TCR) nominally specific for the OVA peptide SIINFEKL (OT-1) reacted to parental Brucella -infected targets as well as OVA-expressing Brucella variants in cytotoxicity assays. Furthermore, splenocytes from Brucella immunized mice produced IFN-γ and exhibited cytotoxicity in response to SIINFEKL-pulsed target cells. To determine if the SIINFEKL-reactive OT-1 TCR could be cross-reacting to Brucella peptides, we searched the Brucella proteome using an algorithm to generate a list of near-neighbor nonamer peptides that would bind to H2K b . Selecting five Brucella peptide candidates, along with controls, we verified that several of these peptides mimicked SIINFEKL resulting in T cell activation through the “SIINFEKL-specific” TCR. Activation was dependent on peptide concentration as well as sequence. Our results underscore the complexity and ubiquity of cross-reactivity in T cell recognition. This cross-reactivity may enable microbes such as Brucella to escape immune surveillance by presenting peptides similar to the host, and may also lead to the activation of autoreactive T cells." @default.
- W2951004074 created "2019-06-27" @default.
- W2951004074 creator A5007144418 @default.
- W2951004074 creator A5010603314 @default.
- W2951004074 creator A5033310887 @default.
- W2951004074 creator A5050137943 @default.
- W2951004074 creator A5054973028 @default.
- W2951004074 creator A5067254212 @default.
- W2951004074 creator A5068140222 @default.
- W2951004074 date "2018-04-14" @default.
- W2951004074 modified "2023-09-23" @default.
- W2951004074 title "Brucella Peptide Cross-Reactive MHC I Presentation Activates SIINFEKL-Specific TCR Expressing T Cells" @default.
- W2951004074 cites W100723737 @default.
- W2951004074 cites W1558078811 @default.
- W2951004074 cites W1819297183 @default.
- W2951004074 cites W1920977578 @default.
- W2951004074 cites W1959677146 @default.
- W2951004074 cites W1967700752 @default.
- W2951004074 cites W1981386085 @default.
- W2951004074 cites W1983760021 @default.
- W2951004074 cites W1986064347 @default.
- W2951004074 cites W1986216579 @default.
- W2951004074 cites W1999361720 @default.
- W2951004074 cites W2035620466 @default.
- W2951004074 cites W2036056711 @default.
- W2951004074 cites W2045209612 @default.
- W2951004074 cites W2047360542 @default.
- W2951004074 cites W2048159226 @default.
- W2951004074 cites W2048784307 @default.
- W2951004074 cites W2049204549 @default.
- W2951004074 cites W2049860314 @default.
- W2951004074 cites W2064747747 @default.
- W2951004074 cites W2071509039 @default.
- W2951004074 cites W2073076889 @default.
- W2951004074 cites W2073578769 @default.
- W2951004074 cites W2074167977 @default.
- W2951004074 cites W2076917860 @default.
- W2951004074 cites W2077372744 @default.
- W2951004074 cites W2084965322 @default.
- W2951004074 cites W2087482245 @default.
- W2951004074 cites W2091814957 @default.
- W2951004074 cites W2093897123 @default.
- W2951004074 cites W2095393450 @default.
- W2951004074 cites W2105381977 @default.
- W2951004074 cites W2113597455 @default.
- W2951004074 cites W2117585409 @default.
- W2951004074 cites W2118393858 @default.
- W2951004074 cites W2120177928 @default.
- W2951004074 cites W2123467717 @default.
- W2951004074 cites W2145850791 @default.
- W2951004074 cites W2146074801 @default.
- W2951004074 cites W2149157363 @default.
- W2951004074 cites W2150632173 @default.
- W2951004074 cites W2152574038 @default.
- W2951004074 cites W2157972078 @default.
- W2951004074 cites W2159878589 @default.
- W2951004074 cites W2160439166 @default.
- W2951004074 cites W2167290112 @default.
- W2951004074 cites W2169145387 @default.
- W2951004074 cites W2186608106 @default.
- W2951004074 cites W2276007012 @default.
- W2951004074 cites W2329836570 @default.
- W2951004074 cites W2395385782 @default.
- W2951004074 cites W2411305131 @default.
- W2951004074 cites W2526466832 @default.
- W2951004074 cites W2605373316 @default.
- W2951004074 cites W2613612207 @default.
- W2951004074 cites W2615707830 @default.
- W2951004074 cites W2618125676 @default.
- W2951004074 cites W2627065887 @default.
- W2951004074 doi "https://doi.org/10.1101/301465" @default.
- W2951004074 hasPublicationYear "2018" @default.
- W2951004074 type Work @default.
- W2951004074 sameAs 2951004074 @default.
- W2951004074 citedByCount "0" @default.
- W2951004074 crossrefType "posted-content" @default.
- W2951004074 hasAuthorship W2951004074A5007144418 @default.
- W2951004074 hasAuthorship W2951004074A5010603314 @default.
- W2951004074 hasAuthorship W2951004074A5033310887 @default.
- W2951004074 hasAuthorship W2951004074A5050137943 @default.
- W2951004074 hasAuthorship W2951004074A5054973028 @default.
- W2951004074 hasAuthorship W2951004074A5067254212 @default.
- W2951004074 hasAuthorship W2951004074A5068140222 @default.
- W2951004074 hasBestOaLocation W29510040741 @default.
- W2951004074 hasConcept C147483822 @default.
- W2951004074 hasConcept C153911025 @default.
- W2951004074 hasConcept C154317977 @default.
- W2951004074 hasConcept C159047783 @default.
- W2951004074 hasConcept C167672396 @default.
- W2951004074 hasConcept C185592680 @default.
- W2951004074 hasConcept C19317047 @default.
- W2951004074 hasConcept C195616568 @default.
- W2951004074 hasConcept C202751555 @default.
- W2951004074 hasConcept C203014093 @default.
- W2951004074 hasConcept C2776090121 @default.
- W2951004074 hasConcept C2776946954 @default.
- W2951004074 hasConcept C2780767784 @default.
- W2951004074 hasConcept C2781429187 @default.
- W2951004074 hasConcept C55493867 @default.
- W2951004074 hasConcept C86803240 @default.