FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951007773> ?p ?o ?g. }

• W2951007773 endingPage "1011" @default.
• W2951007773 startingPage "1003" @default.
• W2951007773 abstract "The present study aimed to establish pretreatment protocols as well as real-time and droplet digital polymerase chain reaction (PCR) methodologies to detect and quantify Erysipelothrix rhusiopathiae (ER) DNA in blood samples from infected chickens, as tools for routine diagnostics and monitoring of experimental infections. Chicken blood is a problematic matrix for PCR analysis because nucleated erythrocytes contribute large amounts of host DNA that inhibit amplification.Using artificially spiked samples of fresh chicken blood, as well as blood samples from three experimental infection studies, the performance of pretreatment protocols, including choice of blood stabilization agent, centrifugation speeds and Ficoll gradient separation, was evaluated. The results were compared with those from traditional culture-based protocols combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS).Results/Key findings. Simple preparations producing cell-free samples performed well on artificial spike-in samples, providing high sensitivity. However, performance was poor in clinical samples or artificial samples where the bacteria were incubated for 4 h or more in fresh blood prior to DNA extraction. In these samples, a Ficoll separation protocol that creates samples rich in lymphocytes, monocytes and thrombocytes prior to DNA extraction was far more effective.Our results indicate that ER bacteria undergo rapid phagocytosis in chicken blood and that analysis of a blood fraction enriched for phagocytic cells is necessary for reliable detection and quantification. The presented results explain the poor performance of PCR detection reported in previously published experimental ER infection studies, and the proposed solutions are likely to have broader implications for PCR-based veterinary diagnostics in non-mammalian host species such as poultry and fish." @default.
• W2951007773 created "2019-06-27" @default.
• W2951007773 creator A5000825732 @default.
• W2951007773 creator A5003849695 @default.
• W2951007773 creator A5013786242 @default.
• W2951007773 creator A5025566596 @default.
• W2951007773 creator A5028807186 @default.
• W2951007773 creator A5054780189 @default.
• W2951007773 creator A5062350357 @default.
• W2951007773 creator A5073223227 @default.
• W2951007773 date "2019-07-01" @default.
• W2951007773 modified "2023-09-25" @default.
• W2951007773 title "Detection and quantification of Erysipelothrix rhusiopathiae in blood from infected chickens – addressing challenges with detection of DNA from infectious agents in host species with nucleated red blood cells" @default.
• W2951007773 cites W126643673 @default.
• W2951007773 cites W1566051882 @default.
• W2951007773 cites W167406457 @default.
• W2951007773 cites W1925986327 @default.
• W2951007773 cites W1982887202 @default.
• W2951007773 cites W1989627556 @default.
• W2951007773 cites W2010425810 @default.
• W2951007773 cites W2012653521 @default.
• W2951007773 cites W2029581977 @default.
• W2951007773 cites W2038358903 @default.
• W2951007773 cites W2043212411 @default.
• W2951007773 cites W2076467367 @default.
• W2951007773 cites W2085116613 @default.
• W2951007773 cites W2091397910 @default.
• W2951007773 cites W2102696040 @default.
• W2951007773 cites W2104180154 @default.
• W2951007773 cites W2112981001 @default.
• W2951007773 cites W2118717769 @default.
• W2951007773 cites W2121774130 @default.
• W2951007773 cites W2128446740 @default.
• W2951007773 cites W2145843770 @default.
• W2951007773 cites W2163568277 @default.
• W2951007773 cites W2439262910 @default.
• W2951007773 cites W2527697804 @default.
• W2951007773 cites W2762246538 @default.
• W2951007773 cites W2789690286 @default.
• W2951007773 cites W2808881184 @default.
• W2951007773 cites W2810233028 @default.
• W2951007773 doi "https://doi.org/10.1099/jmm.0.001016" @default.
• W2951007773 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6939158" @default.
• W2951007773 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31172912" @default.
• W2951007773 hasPublicationYear "2019" @default.
• W2951007773 type Work @default.
• W2951007773 sameAs 2951007773 @default.
• W2951007773 citedByCount "3" @default.
• W2951007773 countsByYear W29510077732020 @default.
• W2951007773 countsByYear W29510077732021 @default.
• W2951007773 countsByYear W29510077732022 @default.
• W2951007773 crossrefType "journal-article" @default.
• W2951007773 hasAuthorship W2951007773A5000825732 @default.
• W2951007773 hasAuthorship W2951007773A5003849695 @default.
• W2951007773 hasAuthorship W2951007773A5013786242 @default.
• W2951007773 hasAuthorship W2951007773A5025566596 @default.
• W2951007773 hasAuthorship W2951007773A5028807186 @default.
• W2951007773 hasAuthorship W2951007773A5054780189 @default.
• W2951007773 hasAuthorship W2951007773A5062350357 @default.
• W2951007773 hasAuthorship W2951007773A5073223227 @default.
• W2951007773 hasBestOaLocation W29510077731 @default.
• W2951007773 hasConcept C104317684 @default.
• W2951007773 hasConcept C153911025 @default.
• W2951007773 hasConcept C1621761 @default.
• W2951007773 hasConcept C185592680 @default.
• W2951007773 hasConcept C203014093 @default.
• W2951007773 hasConcept C2781053203 @default.
• W2951007773 hasConcept C43617362 @default.
• W2951007773 hasConcept C49105822 @default.
• W2951007773 hasConcept C523546767 @default.
• W2951007773 hasConcept C54355233 @default.
• W2951007773 hasConcept C55493867 @default.
• W2951007773 hasConcept C7623868 @default.
• W2951007773 hasConcept C78063203 @default.
• W2951007773 hasConcept C86803240 @default.
• W2951007773 hasConcept C89423630 @default.
• W2951007773 hasConceptScore W2951007773C104317684 @default.
• W2951007773 hasConceptScore W2951007773C153911025 @default.
• W2951007773 hasConceptScore W2951007773C1621761 @default.
• W2951007773 hasConceptScore W2951007773C185592680 @default.
• W2951007773 hasConceptScore W2951007773C203014093 @default.
• W2951007773 hasConceptScore W2951007773C2781053203 @default.
• W2951007773 hasConceptScore W2951007773C43617362 @default.
• W2951007773 hasConceptScore W2951007773C49105822 @default.
• W2951007773 hasConceptScore W2951007773C523546767 @default.
• W2951007773 hasConceptScore W2951007773C54355233 @default.
• W2951007773 hasConceptScore W2951007773C55493867 @default.
• W2951007773 hasConceptScore W2951007773C7623868 @default.
• W2951007773 hasConceptScore W2951007773C78063203 @default.
• W2951007773 hasConceptScore W2951007773C86803240 @default.
• W2951007773 hasConceptScore W2951007773C89423630 @default.
• W2951007773 hasIssue "7" @default.
• W2951007773 hasLocation W29510077731 @default.
• W2951007773 hasLocation W29510077732 @default.
• W2951007773 hasLocation W29510077733 @default.
• W2951007773 hasLocation W29510077734 @default.
• W2951007773 hasLocation W29510077735 @default.
• W2951007773 hasOpenAccess W2951007773 @default.

• next