Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951014987> ?p ?o ?g. }
- W2951014987 abstract "Abstract Antimicrobial peptides (AMPs) are a potential source of new molecules to counter the increase in antimicrobial resistant infections but a better understanding of their properties is required to understand their native function and for effective translation as therapeutics. Details of the mechanism of their interaction with the bacterial plasma membrane are desired since damage or penetration of this structure is considered essential for AMPs activity. Relatively modest modifications to AMPs primary sequence can induce substantial changes in potency and/or spectrum of activity but, hitherto, have not been predicted to substantially alter the mechanism of interaction with the bacterial plasma membrane. Here we use a combination of molecular dynamics simulations, circular dichroism, solid-state NMR and patch clamp to investigate the extent to which temporin B and its analogues can be distinguished both in vitro and in silico on the basis of their interactions with model membranes. Enhancing the hydrophobicity of the N-terminus and cationicity of the C-terminus in temporin B improves its membrane activity and potency against both Gram-negative and Gram-positive bacteria. In contrast, enhancing the cationicity of the N-terminus abrogates its ability to trigger channel conductance and renders it ineffective against Gram-positive bacteria while nevertheless enhancing its potency against Escherichia coli . Our findings suggest even closely related AMPs may target the same bacterium with fundamentally differing mechanisms of action." @default.
- W2951014987 created "2019-06-27" @default.
- W2951014987 creator A5008143288 @default.
- W2951014987 creator A5009492636 @default.
- W2951014987 creator A5016484620 @default.
- W2951014987 creator A5021836407 @default.
- W2951014987 creator A5030510009 @default.
- W2951014987 creator A5045535688 @default.
- W2951014987 creator A5048433029 @default.
- W2951014987 creator A5054821931 @default.
- W2951014987 creator A5062758536 @default.
- W2951014987 creator A5066705302 @default.
- W2951014987 creator A5078941228 @default.
- W2951014987 creator A5082846974 @default.
- W2951014987 creator A5089893497 @default.
- W2951014987 date "2019-02-04" @default.
- W2951014987 modified "2023-10-16" @default.
- W2951014987 title "Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity" @default.
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