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• W2951070060 endingPage "952" @default.
• W2951070060 startingPage "942" @default.
• W2951070060 abstract "Cold storage is commonly employed to delay senescence in ‘Nanguo’ pear after harvest. However, this technique also causes fruit aroma weakening. MicroRNAs are regulators of gene expression at the post-transcriptional level that play important roles in plant development and in eliciting responses to abiotic environmental stressors. In this study, the miRNA transcript profiles of the fruit on the first day (C0, LT0) move in and out of cold storage and the optimum tasting period (COTP, LTOTP) during shelf life at room temperature and after cold storage were analyzed, respectively. 314 known miRNAs were identified in ‘Nanguo’ pear; 176 and 135 miRNAs were significantly differentially expressed on the C0 vs. LT0 and on the COTP vs. LTOTP, respectively. After prediction the target genes of these differentially expressed miRNAs, 9 s-lipoxygenase (LOX2S), 13 s-lipoxygenase (LOX1_5), hydroperoxide lyase (HPL), and alcohol dehydrogenase (ADH1) were found differentially expressed, which were the key genes during aroma formation. The expression pattern of these target genes and the related miRNAs were identified by RT-PCR. mdm-miR172a-h, mdm-miR159a/b, mdm-miR160a-e, mdm-miR395a-i, mdm-miR399a/b/c, mdm/ppe-miR535a/b, and mdm-miR7120a/b may negatively regulate the target genes expression. These results indicate that miRNAs may play key roles in aroma weakening in cold storage ‘Nanguo’ pear and provide valuable information for studying the molecular mechanisms of miRNAs in the aroma weakening of fruit due to low temperature." @default.
• W2951070060 created "2019-06-27" @default.
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• W2951070060 date "2019-02-01" @default.
• W2951070060 modified "2023-09-26" @default.
• W2951070060 title "miRNAs play important roles in aroma weakening during the shelf life of ‘Nanguo’ pear after cold storage" @default.
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• W2951070060 doi "https://doi.org/10.1016/j.foodres.2018.09.031" @default.
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