Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951109098> ?p ?o ?g. }
- W2951109098 abstract "Abstract HIV-1 Rev is an essential viral regulatory protein that facilitates the nuclear export of intron-containing viral mRNAs. It is organized into structured, functionally well-characterized motifs joined by less understood linker regions. Our recent competitive deep mutational scanning study confirmed many known constraints in Rev’s established motifs, but also identified positions of mutational plasticity, most notably in surrounding linker regions. Here, we probe the mutational limits of these linkers by testing the activities of multiple truncation and mass substitution mutations. We find that these regions possess previously unknown structural, functional or regulatory roles, not apparent from systematic point mutational approaches. Specifically, the N- and C-termini of Rev contribute to protein stability; mutations in a turn that connects the two main helices of Rev have different effects in different contexts; and a linker region which connects the second helix of Rev to its nuclear export sequence has structural requirements for function. Thus, Rev function extends beyond its characterized motifs, and is tuned by determinants within seemingly plastic portions of its sequence. Additionally, Rev’s ability to tolerate many of these massive truncations and substitutions illustrates the overall mutational and functional robustness inherent in this viral protein." @default.
- W2951109098 created "2019-06-27" @default.
- W2951109098 creator A5001699296 @default.
- W2951109098 creator A5040482039 @default.
- W2951109098 creator A5049934167 @default.
- W2951109098 creator A5074098242 @default.
- W2951109098 creator A5085879565 @default.
- W2951109098 date "2019-03-26" @default.
- W2951109098 modified "2023-10-16" @default.
- W2951109098 title "Highly Mutable Linker Regions Regulate HIV-1 Rev Function and Stability" @default.
- W2951109098 cites W1804312677 @default.
- W2951109098 cites W1880169023 @default.
- W2951109098 cites W1949686662 @default.
- W2951109098 cites W1968370012 @default.
- W2951109098 cites W1971961101 @default.
- W2951109098 cites W1972715299 @default.
- W2951109098 cites W1972865566 @default.
- W2951109098 cites W1973053780 @default.
- W2951109098 cites W1977013948 @default.
- W2951109098 cites W1977111282 @default.
- W2951109098 cites W1979749509 @default.
- W2951109098 cites W1981457569 @default.
- W2951109098 cites W1985288736 @default.
- W2951109098 cites W1987019783 @default.
- W2951109098 cites W1989498282 @default.
- W2951109098 cites W1992521297 @default.
- W2951109098 cites W1996076856 @default.
- W2951109098 cites W2006784855 @default.
- W2951109098 cites W2015625581 @default.
- W2951109098 cites W2019726061 @default.
- W2951109098 cites W2023487073 @default.
- W2951109098 cites W2025193506 @default.
- W2951109098 cites W2027580775 @default.
- W2951109098 cites W2043148728 @default.
- W2951109098 cites W2051507077 @default.
- W2951109098 cites W2055039476 @default.
- W2951109098 cites W2057132880 @default.
- W2951109098 cites W2063394866 @default.
- W2951109098 cites W2068600036 @default.
- W2951109098 cites W2078188527 @default.
- W2951109098 cites W2082383308 @default.
- W2951109098 cites W2083324135 @default.
- W2951109098 cites W2084131702 @default.
- W2951109098 cites W2089455344 @default.
- W2951109098 cites W2093887574 @default.
- W2951109098 cites W2094650831 @default.
- W2951109098 cites W2097752168 @default.
- W2951109098 cites W2098389839 @default.
- W2951109098 cites W2098838418 @default.
- W2951109098 cites W2101438339 @default.
- W2951109098 cites W2104148336 @default.
- W2951109098 cites W2106659841 @default.
- W2951109098 cites W2107046047 @default.
- W2951109098 cites W2111623022 @default.
- W2951109098 cites W2119408339 @default.
- W2951109098 cites W2124398596 @default.
- W2951109098 cites W2126492433 @default.
- W2951109098 cites W2132629607 @default.
- W2951109098 cites W2139566973 @default.
- W2951109098 cites W2149134545 @default.
- W2951109098 cites W2149145615 @default.
- W2951109098 cites W2165236553 @default.
- W2951109098 cites W2171305498 @default.
- W2951109098 cites W2181261574 @default.
- W2951109098 cites W2198318089 @default.
- W2951109098 cites W2409741307 @default.
- W2951109098 cites W2410492270 @default.
- W2951109098 cites W2425595198 @default.
- W2951109098 cites W2553387043 @default.
- W2951109098 cites W2562684256 @default.
- W2951109098 cites W2580035316 @default.
- W2951109098 cites W2605573414 @default.
- W2951109098 cites W2606937257 @default.
- W2951109098 cites W2625742743 @default.
- W2951109098 cites W2735710385 @default.
- W2951109098 cites W2748006090 @default.
- W2951109098 cites W2788194767 @default.
- W2951109098 cites W2791474337 @default.
- W2951109098 cites W2794966463 @default.
- W2951109098 cites W2802953888 @default.
- W2951109098 cites W2988142046 @default.
- W2951109098 cites W2988945024 @default.
- W2951109098 doi "https://doi.org/10.1038/s41598-019-41582-7" @default.
- W2951109098 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6435700" @default.
- W2951109098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30914719" @default.
- W2951109098 hasPublicationYear "2019" @default.
- W2951109098 type Work @default.
- W2951109098 sameAs 2951109098 @default.
- W2951109098 citedByCount "12" @default.
- W2951109098 countsByYear W29511090982019 @default.
- W2951109098 countsByYear W29511090982020 @default.
- W2951109098 countsByYear W29511090982021 @default.
- W2951109098 countsByYear W29511090982022 @default.
- W2951109098 crossrefType "journal-article" @default.
- W2951109098 hasAuthorship W2951109098A5001699296 @default.
- W2951109098 hasAuthorship W2951109098A5040482039 @default.
- W2951109098 hasAuthorship W2951109098A5049934167 @default.
- W2951109098 hasAuthorship W2951109098A5074098242 @default.
- W2951109098 hasAuthorship W2951109098A5085879565 @default.
- W2951109098 hasBestOaLocation W29511090981 @default.