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- W2951176677 abstract "The influences of N-methyl-N'-nitro-N-nitrosoguanidine (NG) and N-methyl-N- nitrosomethylurea (NMU) on both viability and antibiotic activity of S. sioyaensis Lv81 were investigated. we have determined that the optimal doses of NG and NMU for screening of the clones with increased antibiotic production are those leading to 20-30% and approximately 0,2 % spore survival, respectively. For detection of S. sioyaensis Lv 81 clones with increased level of siomycin biosynthesis, a tipA-based reporter method can be used. It is based on stimulation of the growth of S.lividans TK24 carrying pMO16 plasmid (carries kanamycin/neomycin resistance gene fused to thiopeptide- responsive tipAp promoter) by siomycin on the medium supplemented with kanamycin." @default.
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- W2951176677 date "2009-06-15" @default.
- W2951176677 modified "2023-09-27" @default.
- W2951176677 title "N-METHYL-N'-NITRO-N-NITROSOGUANIDINE AND N-METHYLN-NITROSOMETHYLUREA INFLUENCE ON ANTIBIOTIC ACTIVITY OF STREPTOMYCES SIOYAENSIS LV81" @default.
- W2951176677 doi "https://doi.org/10.18524/2307-4663.2009.2(6).102417" @default.
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