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- W2951330505 abstract "The fluorescent compound, 5(6)-carboxyfluorescein (CF), has been a powerful tool in liposome methodology since the paper by J. N. Weinstein et al. in 1977. Calcein often contains residual fluorescein and other impurities which may interfere with assays such as liposome-cell interactions. A method for estimation of residual fluorescence of fluorescein in calcein is given by H. Diehl. Impurities in calcein do not appear to cause much interference with the use of quenched calcein in liposomes for permeability measurements. Calcein shares all the advantages of 5(6)-CF in liposome methodology and can be substituted for the latter in any of the experiments in which CF has been used. Calcein exhibits fluorescent quenching at neutral pH with transition metals and this is used as the basis of an assay for liposome-liposome fusion. A detailed description of the properties of calcein can be found in a publication by Diehl." @default.
- W2951330505 created "2019-06-27" @default.
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- W2951330505 date "2018-01-18" @default.
- W2951330505 modified "2023-09-25" @default.
- W2951330505 title "Calcein As A Tool In Liposome Methodology" @default.
- W2951330505 cites W1984986786 @default.
- W2951330505 doi "https://doi.org/10.1201/9781351074117-12" @default.
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