Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951493359> ?p ?o ?g. }
- W2951493359 abstract "Abstract Insect odorant receptors show a limited functional expression in various heterologous expression systems including insect and mammalian cells. This may be in part due to the absence of key components driving the release of these proteins from the endoplasmic reticulum and directing them to the plasma membrane. In order to mitigate this problem we took advantage of small export signals within the human HCN1 and Rhodopsin that have been shown to promote protein release from the endoplasmic reticulum and the trafficking of post-Golgi vesicles, respectively. Moreover, we designed a new vector based on a bidirectional expression cassette to drive the functional expression of the insect odorant receptor co-receptor (Orco) and an odor-binding odorant receptor, simultaneously. We show that this new method can be used to reliably express insect odorant receptors in HEK293 cells via transient transfection and that is highly suitable for downstream applications using automated and high-throughput imaging platforms." @default.
- W2951493359 created "2019-06-27" @default.
- W2951493359 creator A5005805159 @default.
- W2951493359 creator A5006718919 @default.
- W2951493359 creator A5026761584 @default.
- W2951493359 creator A5031767592 @default.
- W2951493359 creator A5042681548 @default.
- W2951493359 creator A5065163608 @default.
- W2951493359 creator A5087715963 @default.
- W2951493359 date "2019-06-12" @default.
- W2951493359 modified "2023-10-17" @default.
- W2951493359 title "Optimization of insect odorant receptor trafficking and functional expression via transient transfection in HEK293 cells" @default.
- W2951493359 cites W1968781857 @default.
- W2951493359 cites W1970611538 @default.
- W2951493359 cites W1979758690 @default.
- W2951493359 cites W1982124226 @default.
- W2951493359 cites W1988715808 @default.
- W2951493359 cites W2002141216 @default.
- W2951493359 cites W2004912143 @default.
- W2951493359 cites W2005111341 @default.
- W2951493359 cites W2008975313 @default.
- W2951493359 cites W2033111008 @default.
- W2951493359 cites W2059175123 @default.
- W2951493359 cites W2060192444 @default.
- W2951493359 cites W2062618421 @default.
- W2951493359 cites W2065390054 @default.
- W2951493359 cites W2078997561 @default.
- W2951493359 cites W2082788644 @default.
- W2951493359 cites W2086323500 @default.
- W2951493359 cites W2087656949 @default.
- W2951493359 cites W2093902047 @default.
- W2951493359 cites W2094307240 @default.
- W2951493359 cites W2097117811 @default.
- W2951493359 cites W2110450887 @default.
- W2951493359 cites W2115542528 @default.
- W2951493359 cites W2118200380 @default.
- W2951493359 cites W2119960283 @default.
- W2951493359 cites W2129054103 @default.
- W2951493359 cites W2142523086 @default.
- W2951493359 cites W2143733947 @default.
- W2951493359 cites W2152550792 @default.
- W2951493359 cites W2159267296 @default.
- W2951493359 cites W2167279371 @default.
- W2951493359 cites W2188402828 @default.
- W2951493359 cites W2212640525 @default.
- W2951493359 cites W2217392873 @default.
- W2951493359 cites W2312329157 @default.
- W2951493359 cites W2327289347 @default.
- W2951493359 cites W2339822075 @default.
- W2951493359 cites W2340102874 @default.
- W2951493359 cites W2408576746 @default.
- W2951493359 cites W2415260792 @default.
- W2951493359 cites W2473988462 @default.
- W2951493359 cites W2580035316 @default.
- W2951493359 cites W2749120804 @default.
- W2951493359 cites W2793536147 @default.
- W2951493359 cites W2888726474 @default.
- W2951493359 cites W2900597178 @default.
- W2951493359 cites W3102669448 @default.
- W2951493359 cites W4242289937 @default.
- W2951493359 cites W2492185198 @default.
- W2951493359 doi "https://doi.org/10.1101/669127" @default.
- W2951493359 hasPublicationYear "2019" @default.
- W2951493359 type Work @default.
- W2951493359 sameAs 2951493359 @default.
- W2951493359 citedByCount "0" @default.
- W2951493359 crossrefType "posted-content" @default.
- W2951493359 hasAuthorship W2951493359A5005805159 @default.
- W2951493359 hasAuthorship W2951493359A5006718919 @default.
- W2951493359 hasAuthorship W2951493359A5026761584 @default.
- W2951493359 hasAuthorship W2951493359A5031767592 @default.
- W2951493359 hasAuthorship W2951493359A5042681548 @default.
- W2951493359 hasAuthorship W2951493359A5065163608 @default.
- W2951493359 hasAuthorship W2951493359A5087715963 @default.
- W2951493359 hasBestOaLocation W29514933591 @default.
- W2951493359 hasConcept C104317684 @default.
- W2951493359 hasConcept C108305142 @default.
- W2951493359 hasConcept C119062480 @default.
- W2951493359 hasConcept C158617107 @default.
- W2951493359 hasConcept C170493617 @default.
- W2951493359 hasConcept C174510640 @default.
- W2951493359 hasConcept C40767141 @default.
- W2951493359 hasConcept C54009773 @default.
- W2951493359 hasConcept C55493867 @default.
- W2951493359 hasConcept C86803240 @default.
- W2951493359 hasConcept C95444343 @default.
- W2951493359 hasConceptScore W2951493359C104317684 @default.
- W2951493359 hasConceptScore W2951493359C108305142 @default.
- W2951493359 hasConceptScore W2951493359C119062480 @default.
- W2951493359 hasConceptScore W2951493359C158617107 @default.
- W2951493359 hasConceptScore W2951493359C170493617 @default.
- W2951493359 hasConceptScore W2951493359C174510640 @default.
- W2951493359 hasConceptScore W2951493359C40767141 @default.
- W2951493359 hasConceptScore W2951493359C54009773 @default.
- W2951493359 hasConceptScore W2951493359C55493867 @default.
- W2951493359 hasConceptScore W2951493359C86803240 @default.
- W2951493359 hasConceptScore W2951493359C95444343 @default.
- W2951493359 hasLocation W29514933591 @default.
- W2951493359 hasLocation W29514933592 @default.
- W2951493359 hasLocation W29514933593 @default.