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• W2951534411 abstract "We developed the engineered DNA ‐binding molecule‐mediated chromatin immunoprecipitation (enCh IP ) technology to isolate specific genomic regions while retaining their molecular interactions. In enCh IP , the locus of interest is tagged with an engineered DNA ‐binding molecule, such as a modified form of the clustered regularly interspaced short palindromic repeats ( CRISPR ) system containing a guide RNA ( gRNA ) and a catalytically inactive form of Cas9 ( dC as9). The locus is then affinity‐purified to enable identification of associated molecules. In this study, we generated transgenic mice expressing 3x FLAG ‐tagged Streptococcus pyogenes dC as9 (3x FLAG ‐ dC as9) and retrovirally transduced gRNA into primary CD 4 + T cells from these mice for enCh IP . Using this approach, we achieved high yields of enCh IP at the targeted genomic region. Our novel transgenic mouse lines provide a valuable tool for enCh IP analysis in primary mouse cells." @default.
• W2951534411 created "2019-06-27" @default.
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• W2951534411 date "2018-02-26" @default.
• W2951534411 modified "2023-10-11" @default.
• W2951534411 title "Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis" @default.
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• W2951534411 doi "https://doi.org/10.1111/gtc.12573" @default.
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• W2951534411 hasPublicationYear "2018" @default.
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