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• W2951624209 abstract "Abstract Accurate manipulation of metabolites in the monolignol biosynthetic pathway is a key step for controlling lignin content, structure, and other wood properties important to the bioenergy and biomaterial industries. A crucial component of this strategy is predicting how single and combinatorial knockdowns of monolignol specific gene transcripts influence the abundance of monolignol proteins, which are the driving mechanisms of monolignol biosynthesis. Computational models have been developed to estimate protein abundances from transcript perturbations of monolignol specific genes. The accuracy of these models, however, is hindered by the inability to capture indirect regulatory influences on other pathway genes. Here, we examine the manifestation of these indirect influences collectively on transgenic transcript and protein abundances, identifying putative indirect regulatory influences that occur when one or more specific monolignol pathway genes are perturbed. We created a computational model using sparse maximum likelihood to estimate the resulting monolignol transcript and protein abundances in transgenic Populus trichocarpa based on desired single or combinatorial knockdowns of specific monolignol genes. Using in-silico simulations of this model and root mean square error, we show that our model more accurately estimates transcript and protein abundances in differentiating xylem tissue when individual and families of monolignol genes were perturbed. This approach provides a useful computational tool for exploring the cascaded impact of single and combinatorial modifications of monolignol specific genes on lignin and other wood properties. Additionally, these results can be used to guide future experiments to elucidate the mechanisms responsible for the indirect influences. Author summary Engineering trees to have desirable lignin and wood traits is of significant interest to the bioenergy and biomaterial industries. Genetically modifying the expression of the genes that drive the monolignol biosynthetic pathway is a useful method for obtaining new traits. Modifying the expression of one gene affects not only the abundance of its encoded protein, but can also indirectly impact the amount of other transcripts and proteins. These proteins drive the monolignol biosynthetic pathway. Having an accurate representation of their abundances is key to understanding how lignin and wood traits are altered. We developed a computational model to estimate how the abundance of monolignol transcripts and proteins are changed when one or more monolignol genes are knocked down. Specifying only the abundances of the targeted genes as input, our model estimates how the levels of the other, untargeted, transcripts and proteins are altered. Our model captures indirect regulatory influences at the transcript and protein levels observed in experimental data. The model is an important addition to current models of lignin biosynthesis. By incorporating our approach into the existing models, we expect to improve our ability to explore how new combinations of gene knockdowns impact lignin and many other wood properties." @default.
• W2951624209 created "2019-06-27" @default.
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• W2951624209 date "2019-06-19" @default.
• W2951624209 modified "2023-09-26" @default.
• W2951624209 title "Modeling cross-regulatory influences on monolignol transcripts and proteins under single and combinatorial gene knockdowns in Populus trichocarpa" @default.
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• W2951624209 doi "https://doi.org/10.1101/677047" @default.
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