FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951720007> ?p ?o ?g. }

• W2951720007 endingPage "113208" @default.
• W2951720007 startingPage "113208" @default.
• W2951720007 abstract "We developed a specific competitive enzyme-linked immunosorbent assay (ELISA) for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone (FSH). We previously produced a full-length single chain recombinant yellowtail kingfish FSH using the Pichia pastoris expression system. We used the same method to produce the β subunit of the hormone, against which polyclonal antibodies were raised in rabbits. We first confirmed immunoreactivity of the polyclonal antibodies with the recombinant full length FSH and FSHβ as well as plasma and pituitary FSH of sexually immature and mature yellowtail kingfish by Western blot analysis. We then developed a precise and reproducible ELISA for yellowtail kingfish FSH and validated the assay in plasma and pituitary extracts. The intra- and inter-assay coefficients of variation was <2.2% and 10.2%, respectively. The sensitivity of the assay was 78 pg/ml. For further validation of the assay, we measured the plasma FSH in immature yellowtail kingfish treated with increasing doses (blank, 50, 100 and 150 µg/kg) of kisseptin2-10 peptide from a previous study. The dose response observed in treated females was not significant, however the increased plasma FSH levels coincided with the significantly higher estradiol levels we previously reported in the treated groups. We assessed the applicability of the assay in measuring circulating FSH in other species. We observed parallelism between the linearized FSH standard curve and displacement curves of serially diluted plasma from Atlantic bluefin tuna (Thunnus thynnus) and tilapia (Oreochromis niloticus). We also observed similar parallelism with full length recombinant giant grouper (Epinephelus lanceolatus) FSH. The ELISA we developed for yellowtail kingfish FSH will be useful in understanding the reproductive biology of the species as well as enhancing its aquaculture." @default.
• W2951720007 created "2019-06-27" @default.
• W2951720007 creator A5015630131 @default.
• W2951720007 creator A5023303357 @default.
• W2951720007 creator A5044485297 @default.
• W2951720007 creator A5049218066 @default.
• W2951720007 creator A5062482023 @default.
• W2951720007 creator A5080239648 @default.
• W2951720007 date "2019-10-01" @default.
• W2951720007 modified "2023-10-03" @default.
• W2951720007 title "Development of specific enzyme-linked immunosorbent assay for yellowtail kingfish (Seriola lalandi) follicle stimulating hormone using recombinant gonadotropins" @default.
• W2951720007 cites W1844112381 @default.
• W2951720007 cites W1968863496 @default.
• W2951720007 cites W1970691941 @default.
• W2951720007 cites W1971339178 @default.
• W2951720007 cites W1981781900 @default.
• W2951720007 cites W2000189523 @default.
• W2951720007 cites W2001542067 @default.
• W2951720007 cites W2001575169 @default.
• W2951720007 cites W2002526474 @default.
• W2951720007 cites W2038428130 @default.
• W2951720007 cites W2047663697 @default.
• W2951720007 cites W2057213891 @default.
• W2951720007 cites W2057223558 @default.
• W2951720007 cites W2064904457 @default.
• W2951720007 cites W2064976241 @default.
• W2951720007 cites W2068207925 @default.
• W2951720007 cites W2081941747 @default.
• W2951720007 cites W2098181334 @default.
• W2951720007 cites W2102859380 @default.
• W2951720007 cites W2103056296 @default.
• W2951720007 cites W2111676948 @default.
• W2951720007 cites W2123944209 @default.
• W2951720007 cites W2125629855 @default.
• W2951720007 cites W2129380448 @default.
• W2951720007 cites W2150623204 @default.
• W2951720007 cites W2164892339 @default.
• W2951720007 cites W2166445653 @default.
• W2951720007 cites W2166626204 @default.
• W2951720007 cites W2235597648 @default.
• W2951720007 cites W2293737363 @default.
• W2951720007 cites W235629129 @default.
• W2951720007 cites W2519972072 @default.
• W2951720007 cites W2566037439 @default.
• W2951720007 cites W2601668927 @default.
• W2951720007 cites W2766466525 @default.
• W2951720007 cites W2769807787 @default.
• W2951720007 cites W2801075051 @default.
• W2951720007 cites W2811063449 @default.
• W2951720007 cites W2905357742 @default.
• W2951720007 doi "https://doi.org/10.1016/j.ygcen.2019.113208" @default.
• W2951720007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31226255" @default.
• W2951720007 hasPublicationYear "2019" @default.
• W2951720007 type Work @default.
• W2951720007 sameAs 2951720007 @default.
• W2951720007 citedByCount "7" @default.
• W2951720007 countsByYear W29517200072019 @default.
• W2951720007 countsByYear W29517200072020 @default.
• W2951720007 countsByYear W29517200072021 @default.
• W2951720007 countsByYear W29517200072022 @default.
• W2951720007 countsByYear W29517200072023 @default.
• W2951720007 crossrefType "journal-article" @default.
• W2951720007 hasAuthorship W2951720007A5015630131 @default.
• W2951720007 hasAuthorship W2951720007A5023303357 @default.
• W2951720007 hasAuthorship W2951720007A5044485297 @default.
• W2951720007 hasAuthorship W2951720007A5049218066 @default.
• W2951720007 hasAuthorship W2951720007A5062482023 @default.
• W2951720007 hasAuthorship W2951720007A5080239648 @default.
• W2951720007 hasConcept C104317684 @default.
• W2951720007 hasConcept C126322002 @default.
• W2951720007 hasConcept C134018914 @default.
• W2951720007 hasConcept C159654299 @default.
• W2951720007 hasConcept C16685009 @default.
• W2951720007 hasConcept C203014093 @default.
• W2951720007 hasConcept C2778575703 @default.
• W2951720007 hasConcept C2779279165 @default.
• W2951720007 hasConcept C2780532849 @default.
• W2951720007 hasConcept C2781372317 @default.
• W2951720007 hasConcept C2909208804 @default.
• W2951720007 hasConcept C40767141 @default.
• W2951720007 hasConcept C505870484 @default.
• W2951720007 hasConcept C55493867 @default.
• W2951720007 hasConcept C71315377 @default.
• W2951720007 hasConcept C71924100 @default.
• W2951720007 hasConcept C86803240 @default.
• W2951720007 hasConcept C956191 @default.
• W2951720007 hasConceptScore W2951720007C104317684 @default.
• W2951720007 hasConceptScore W2951720007C126322002 @default.
• W2951720007 hasConceptScore W2951720007C134018914 @default.
• W2951720007 hasConceptScore W2951720007C159654299 @default.
• W2951720007 hasConceptScore W2951720007C16685009 @default.
• W2951720007 hasConceptScore W2951720007C203014093 @default.
• W2951720007 hasConceptScore W2951720007C2778575703 @default.
• W2951720007 hasConceptScore W2951720007C2779279165 @default.
• W2951720007 hasConceptScore W2951720007C2780532849 @default.
• W2951720007 hasConceptScore W2951720007C2781372317 @default.
• W2951720007 hasConceptScore W2951720007C2909208804 @default.
• W2951720007 hasConceptScore W2951720007C40767141 @default.

• next