FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951846114> ?p ?o ?g. }

• W2951846114 endingPage "109855" @default.
• W2951846114 startingPage "109855" @default.
• W2951846114 abstract "Identification of key components in the chemical and physical milieu for directing osteogenesis is a requirement in the investigation of tissue engineering scaffolds for advancement of bone regeneration. In this study, we engineered different gelatin-based cryogels and studied the effect of nanohydroxyapatite (nHAP) and crosslinking agents on scaffold properties and its osteogenic response towards bone marrow stem cells (BMSCs). The cryogels examined are 5% gelatin and 5% gelatin/2.5% nHAP, crosslinked either with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) or glutaraldehyde (GA). We confirmed that nHAP or the crosslinking agent has no effects on scaffold pore size and porosity. Nonetheless, incorporation of nHAP increased mechanical strength, swelling ratio and degree of crosslinking, but decreased degradation rate. Cryogels crosslinked with EDC showed faster degradation and promoted osteogenic differentiation of BMSCs while those prepared from GA crosslinking promoted proliferation of BMSCs. Furthermore, osteogenic differentiation was always enhanced in the presence of nHAP irrespective of the culture medium (normal or osteogenic) used but osteogenic medium always provide a higher extent of osteogenic differentiation. Employing gelatin/nHAP cryogel crosslinked by EDC in a bioreactor for dynamic culture of BMSCs, cyclic compressive mechanical simulation was found to be beneficial for both cell proliferation and osteogenic differentiation. However, the optimum conditions for osteogenic differentiation and cell proliferation were found at 30% and 60% strain, respectively. We thus demonstrated that osteogenic differentiation of BMSCs could be tuned by taking advantages of chemical cues generated from scaffold chemistry or physical cues generated from dynamic cell culture in vitro. Furthermore, by combining the best cryogel preparation and in vitro cell culture condition for osteogenesis, we successfully employed in vitro cultured cryogel/BMSCs constructs for repair of rabbit critical-sized cranial bone defects." @default.
• W2951846114 created "2019-06-27" @default.
• W2951846114 creator A5004537785 @default.
• W2951846114 creator A5018529828 @default.
• W2951846114 creator A5022495437 @default.
• W2951846114 creator A5028998292 @default.
• W2951846114 creator A5046730757 @default.
• W2951846114 creator A5055381437 @default.
• W2951846114 creator A5058099957 @default.
• W2951846114 date "2019-11-01" @default.
• W2951846114 modified "2023-10-17" @default.
• W2951846114 title "Rational design of gelatin/nanohydroxyapatite cryogel scaffolds for bone regeneration by introducing chemical and physical cues to enhance osteogenesis of bone marrow mesenchymal stem cells" @default.
• W2951846114 cites W1202974101 @default.
• W2951846114 cites W1498802694 @default.
• W2951846114 cites W160482963 @default.
• W2951846114 cites W1832982627 @default.
• W2951846114 cites W1952486103 @default.
• W2951846114 cites W1964842639 @default.
• W2951846114 cites W1972451730 @default.
• W2951846114 cites W1976296670 @default.
• W2951846114 cites W1984844893 @default.
• W2951846114 cites W1984971493 @default.
• W2951846114 cites W1987529308 @default.
• W2951846114 cites W1991162692 @default.
• W2951846114 cites W1991956475 @default.
• W2951846114 cites W2001496248 @default.
• W2951846114 cites W2009119064 @default.
• W2951846114 cites W2010168934 @default.
• W2951846114 cites W2011417733 @default.
• W2951846114 cites W2013035214 @default.
• W2951846114 cites W2016519321 @default.
• W2951846114 cites W2018474760 @default.
• W2951846114 cites W2024261809 @default.
• W2951846114 cites W2035469813 @default.
• W2951846114 cites W2037810763 @default.
• W2951846114 cites W2041811569 @default.
• W2951846114 cites W2042258668 @default.
• W2951846114 cites W2052647448 @default.
• W2951846114 cites W2055037957 @default.
• W2951846114 cites W2061108278 @default.
• W2951846114 cites W2067595293 @default.
• W2951846114 cites W2071345032 @default.
• W2951846114 cites W2071567817 @default.
• W2951846114 cites W2073872114 @default.
• W2951846114 cites W2082358669 @default.
• W2951846114 cites W2084610464 @default.
• W2951846114 cites W2085481239 @default.
• W2951846114 cites W2087037684 @default.
• W2951846114 cites W2087644457 @default.
• W2951846114 cites W2100132015 @default.
• W2951846114 cites W2103630920 @default.
• W2951846114 cites W2104169183 @default.
• W2951846114 cites W2107737415 @default.
• W2951846114 cites W2110200535 @default.
• W2951846114 cites W2121292799 @default.
• W2951846114 cites W2127867513 @default.
• W2951846114 cites W2129360703 @default.
• W2951846114 cites W2144971839 @default.
• W2951846114 cites W2152723687 @default.
• W2951846114 cites W2156611734 @default.
• W2951846114 cites W2188868789 @default.
• W2951846114 cites W2216626824 @default.
• W2951846114 cites W2225175924 @default.
• W2951846114 cites W2274322850 @default.
• W2951846114 cites W2329606456 @default.
• W2951846114 cites W2344589655 @default.
• W2951846114 cites W2461589590 @default.
• W2951846114 cites W2555116909 @default.
• W2951846114 cites W2555570490 @default.
• W2951846114 cites W2746574475 @default.
• W2951846114 cites W2765510394 @default.
• W2951846114 cites W2769583695 @default.
• W2951846114 cites W2774085282 @default.
• W2951846114 cites W2791297379 @default.
• W2951846114 cites W33514103 @default.
• W2951846114 doi "https://doi.org/10.1016/j.msec.2019.109855" @default.
• W2951846114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31500067" @default.
• W2951846114 hasPublicationYear "2019" @default.
• W2951846114 type Work @default.
• W2951846114 sameAs 2951846114 @default.
• W2951846114 citedByCount "37" @default.
• W2951846114 countsByYear W29518461142020 @default.
• W2951846114 countsByYear W29518461142021 @default.
• W2951846114 countsByYear W29518461142022 @default.
• W2951846114 countsByYear W29518461142023 @default.
• W2951846114 crossrefType "journal-article" @default.
• W2951846114 hasAuthorship W2951846114A5004537785 @default.
• W2951846114 hasAuthorship W2951846114A5018529828 @default.
• W2951846114 hasAuthorship W2951846114A5022495437 @default.
• W2951846114 hasAuthorship W2951846114A5028998292 @default.
• W2951846114 hasAuthorship W2951846114A5046730757 @default.
• W2951846114 hasAuthorship W2951846114A5055381437 @default.
• W2951846114 hasAuthorship W2951846114A5058099957 @default.
• W2951846114 hasConcept C12554922 @default.
• W2951846114 hasConcept C136229726 @default.
• W2951846114 hasConcept C171056886 @default.
• W2951846114 hasConcept C185592680 @default.
• W2951846114 hasConcept C192562407 @default.

• next