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- W2951879551 abstract "Marchantia polymorpha is one of the model species of basal land plants. Although CRISPR/Cas9-based genome editing has already been demonstrated for this plant, the efficiency was too low to apply to functional analysis. In this study, we show the establishment of CRISPR/Cas9 genome editing vectors with high efficiency for both construction and genome editing. Codon optimization of Cas9 to Arabidopsis achieved over 70% genome editing efficiency at two loci tested. Systematic assessment revealed that guide sequences of 17 nt or shorter dramatically decreased this efficiency. We also demonstrated that a combinatorial use of this system and a floxed complementation construct enabled conditional analysis of a nearly essential gene. This study reports that simple, rapid, and efficient genome editing is feasible with the series of developed vectors." @default.
- W2951879551 created "2019-06-27" @default.
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- W2951879551 date "2018-10-31" @default.
- W2951879551 modified "2023-10-10" @default.
- W2951879551 title "Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha" @default.
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- W2951879551 doi "https://doi.org/10.1371/journal.pone.0205117" @default.
- W2951879551 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6209168" @default.
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