FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2951885103> ?p ?o ?g. }

• W2951885103 endingPage "900" @default.
• W2951885103 startingPage "900" @default.
• W2951885103 abstract "Background: Interleukin-1b (IL-1b) is not only a key factor of inflammation, but also a participant in regulation of hematopoietic stem cells (HSCs) and stromal microenvironment. It is known that IL-1b affect self-renewal of HSCs and drives their differentiation towards myeloid progenitors. At the same time, IL-1b promotes expansion of hematopoietic territory by stimulating proliferation and differentiation of stromal precursor cells. This can be achieved upon irradiation, which induces IL-1b expression in bones. The elevated level of IL-1b persists a prolonged time until death of irradiated animal. One of possible explanations of this phenomenon could be demethylation of CpG dinucleotides in Il1b promoter. Aims: The aim of this study was to determine if ionizing radiation can trigger alterations in Il1b promoter and result in stable changes in its expression as a consequence. Methods: CBF1 mice (C57Bl/6J x CBA) were irradiated at a dose of 6 Gy with cobalt-60 source. After 1, 7, 60, 270 and 600 days after irradiation, DNA was isolated from the bones of irradiated and control mice. Bisulfite conversion of DNA was performed followed by PCR. The PCR product was cloned into a plasmid and competent E. coli cells were transformed. Individual colonies of transformants with the insert were selected by blue-white test. DNA was isolated from 15-25 white colonies and the insert sequence was determined by Sanger sequencing. The extent of CpG methylation in the initial cell population was determined by the ratio of colonies with the C or T signal in the positions corresponding to CpG. The extent of CpG methylation in the Il1b promoter was also determined in the total cell population by direct sequencing of the converted PCR product. Results: It was shown that, in general, the CpG methylation profile in the Il1b promoter does not change after irradiation. Both in the control and in the experimental group, CpGs in the proximal (-154 – -379 bp from the start of transcription) and in the distant (-3324 – -3629 bp) regions were most methylated (the ratio of methylated CpGs = 0.7-0.9). On the contrary, CpG, located in the middle (-2152 – -2490 bp), were the least methylated (the ratio of methylated < 0.6). A statistically significant decrease in methylation of individual cytosines was detected after 60 days (-317 bp) and 600 days (-194 bp) after irradiation, however, these changes were unstable and were not observed for other periods after irradiation. The functional significance of these changes remains to be established. This study demonstrated that ionizing radiation can alter the methylation of individual CpGs in gene promoters. Summary/Conclusion: It is shown that irradiation can lead to alterations in the methylation profile of the Il1b promoter in bone cells, which can exert influence on the functioning and maintenance of HSCs within osteoblastic niches. This may affect the quality of hematopoiesis and the regulation of inflammatory processes. Probably, hypomethylation agents used to treat patients with hematological malignancies affect not only tumor cells, but also cells of the hematopoietic microenvironment." @default.
• W2951885103 created "2019-06-27" @default.
• W2951885103 creator A5007518477 @default.
• W2951885103 creator A5030795526 @default.
• W2951885103 creator A5080456087 @default.
• W2951885103 creator A5089672253 @default.
• W2951885103 date "2019-06-01" @default.
• W2951885103 modified "2023-09-24" @default.
• W2951885103 title "PB1985 THE INFLUENCE OF IL1B PROMOTER METHYLATION PROFILE ON FUNCTIONAL STATE OF HEMATOPOIETIC MICROENVIRONMENT" @default.
• W2951885103 doi "https://doi.org/10.1097/01.hs9.0000566432.97331.a0" @default.
• W2951885103 hasPublicationYear "2019" @default.
• W2951885103 type Work @default.
• W2951885103 sameAs 2951885103 @default.
• W2951885103 citedByCount "0" @default.
• W2951885103 crossrefType "journal-article" @default.
• W2951885103 hasAuthorship W2951885103A5007518477 @default.
• W2951885103 hasAuthorship W2951885103A5030795526 @default.
• W2951885103 hasAuthorship W2951885103A5080456087 @default.
• W2951885103 hasAuthorship W2951885103A5089672253 @default.
• W2951885103 hasBestOaLocation W29518851031 @default.
• W2951885103 hasConcept C104317684 @default.
• W2951885103 hasConcept C109159458 @default.
• W2951885103 hasConcept C117262875 @default.
• W2951885103 hasConcept C140173407 @default.
• W2951885103 hasConcept C1491633281 @default.
• W2951885103 hasConcept C150194340 @default.
• W2951885103 hasConcept C153911025 @default.
• W2951885103 hasConcept C16824249 @default.
• W2951885103 hasConcept C16930146 @default.
• W2951885103 hasConcept C189235521 @default.
• W2951885103 hasConcept C190727270 @default.
• W2951885103 hasConcept C201750760 @default.
• W2951885103 hasConcept C203014093 @default.
• W2951885103 hasConcept C2780007613 @default.
• W2951885103 hasConcept C28328180 @default.
• W2951885103 hasConcept C2908647359 @default.
• W2951885103 hasConcept C33288867 @default.
• W2951885103 hasConcept C502942594 @default.
• W2951885103 hasConcept C54355233 @default.
• W2951885103 hasConcept C552990157 @default.
• W2951885103 hasConcept C71924100 @default.
• W2951885103 hasConcept C86803240 @default.
• W2951885103 hasConcept C95444343 @default.
• W2951885103 hasConcept C99454951 @default.
• W2951885103 hasConceptScore W2951885103C104317684 @default.
• W2951885103 hasConceptScore W2951885103C109159458 @default.
• W2951885103 hasConceptScore W2951885103C117262875 @default.
• W2951885103 hasConceptScore W2951885103C140173407 @default.
• W2951885103 hasConceptScore W2951885103C1491633281 @default.
• W2951885103 hasConceptScore W2951885103C150194340 @default.
• W2951885103 hasConceptScore W2951885103C153911025 @default.
• W2951885103 hasConceptScore W2951885103C16824249 @default.
• W2951885103 hasConceptScore W2951885103C16930146 @default.
• W2951885103 hasConceptScore W2951885103C189235521 @default.
• W2951885103 hasConceptScore W2951885103C190727270 @default.
• W2951885103 hasConceptScore W2951885103C201750760 @default.
• W2951885103 hasConceptScore W2951885103C203014093 @default.
• W2951885103 hasConceptScore W2951885103C2780007613 @default.
• W2951885103 hasConceptScore W2951885103C28328180 @default.
• W2951885103 hasConceptScore W2951885103C2908647359 @default.
• W2951885103 hasConceptScore W2951885103C33288867 @default.
• W2951885103 hasConceptScore W2951885103C502942594 @default.
• W2951885103 hasConceptScore W2951885103C54355233 @default.
• W2951885103 hasConceptScore W2951885103C552990157 @default.
• W2951885103 hasConceptScore W2951885103C71924100 @default.
• W2951885103 hasConceptScore W2951885103C86803240 @default.
• W2951885103 hasConceptScore W2951885103C95444343 @default.
• W2951885103 hasConceptScore W2951885103C99454951 @default.
• W2951885103 hasIssue "S1" @default.
• W2951885103 hasLocation W29518851031 @default.
• W2951885103 hasLocation W29518851032 @default.
• W2951885103 hasOpenAccess W2951885103 @default.
• W2951885103 hasPrimaryLocation W29518851031 @default.
• W2951885103 hasRelatedWork W1970892827 @default.
• W2951885103 hasRelatedWork W1980393350 @default.
• W2951885103 hasRelatedWork W1982107116 @default.
• W2951885103 hasRelatedWork W2034231219 @default.
• W2951885103 hasRelatedWork W2074144170 @default.
• W2951885103 hasRelatedWork W2098566626 @default.
• W2951885103 hasRelatedWork W2154669051 @default.
• W2951885103 hasRelatedWork W2417345120 @default.
• W2951885103 hasRelatedWork W2550885802 @default.
• W2951885103 hasRelatedWork W3034064495 @default.
• W2951885103 hasVolume "3" @default.
• W2951885103 isParatext "false" @default.
• W2951885103 isRetracted "false" @default.
• W2951885103 magId "2951885103" @default.
• W2951885103 workType "article" @default.