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• W2951997564 abstract "Abstract In eukaryotes, several “hub” proteins integrate signals from different interacting partners that bind through intrinsically disordered regions. The 14-3-3 protein hub, which plays wide-ranging roles in cellular processes, has been linked to numerous human disorders and is a promising target for therapeutic intervention. Partner proteins usually bind via insertion of a phosphopeptide into an amphipathic groove of 14-3-3. Structural plasticity in the groove generates promiscuity allowing accommodation of hundreds of different partners. So far, accurate structural information has been derived for only a few 14-3-3 complexes with phosphopeptide-containing proteins and a variety of complexes with short synthetic peptides. To further advance structural studies, here we propose a novel approach based on fusing 14-3-3 proteins with the target partner peptide sequences. Such chimeric proteins are easy to design, express, purify and crystallize. Peptide attachment to the C terminus of 14-3-3 via an optimal linker allows its phosphorylation by protein kinase A during bacterial co-expression and subsequent binding at the amphipathic groove. Crystal structures of 14-3-3 chimeras with three different peptides provide detailed structural information on peptide-14-3-3 interactions. This simple but powerful approach, employing chimeric proteins, can reinvigorate studies of 14-3-3/phosphoprotein assemblies, including those with challenging low-affinity partners, and may facilitate the design of novel biosensors." @default.
• W2951997564 created "2019-06-27" @default.
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• W2951997564 date "2017-09-20" @default.
• W2951997564 modified "2023-10-16" @default.
• W2951997564 title "Chimeric 14-3-3 proteins for unraveling interactions with intrinsically disordered partners" @default.
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• W2951997564 doi "https://doi.org/10.1038/s41598-017-12214-9" @default.
• W2951997564 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5607241" @default.
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• W2951997564 hasPublicationYear "2017" @default.
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