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- W2952219476 abstract "Many of the biological functions of a cell are dictated by the intricate motion of proteins within its membrane over a spatial range of nanometres to tens of micrometres and time intervals of microseconds to minutes. This rich parameter space is not accessible by fluorescence microscopy, but it is within reach of interferometric scattering (iSCAT) particle tracking. However, as iSCAT is sensitive even to single unlabelled proteins, it is often accompanied by a large speckle-like background, which poses a substantial challenge for its application to cellular imaging. Here, we employ a new image processing approach to overcome this difficulty and demonstrate tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes. We provide examples of nanoscale motion and confinement in ubiquitous processes such as diffusion in the plasma membrane, transport on filopodia and rotational motion during endocytosis. Interferometric scattering microscopy is employed to track proteins in live cell membranes, demonstrating tracking of transmembrane epidermal growth factor receptors with nanometre precision in all three dimensions at up to microsecond speeds and for durations of tens of minutes." @default.
- W2952219476 created "2019-06-27" @default.
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- W2952219476 date "2019-04-15" @default.
- W2952219476 modified "2023-10-01" @default.
- W2952219476 title "Interferometric scattering microscopy reveals microsecond nanoscopic protein motion on a live cell membrane" @default.
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- W2952219476 doi "https://doi.org/10.1038/s41566-019-0414-6" @default.
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