FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952276039> ?p ?o ?g. }

• W2952276039 endingPage "109061" @default.
• W2952276039 startingPage "109061" @default.
• W2952276039 abstract "This study aimed to explore the effects of hypoxia and acid-sensing ion channel 3 (ASIC3) on nucleus pulposus cells from cell behavior to molecular mechanism. Primary rabbit nucleus pulposus cells were isolated and identified by HE, toluidine blue and immunohistochemical staining of collagen II. 2% O2 and 48 h were screened as optimal oxygen concentration and effect time, respectively, by determining cell apoptosis and mRNA expression of ASIC3, hypoxia inducible factor-1α (HIF-1α) and aquaporin 3. FLuo-3 AM labeling showed that the Ca2+ concentration in cells increased under hypoxia condition. shRNA-ASIC3 and ASIC3 expression vector were transfected into cells. Subsequently, cells were divided into six groups: Control, 2% O2, shRNA-NC+2% O2, shRNA-ASIC3 + 2% O2, Vector+2% O2 and ASIC3 + 2% O2. Flow cytometry, CCK-8 assay, transmission electron microscopy, immunofluorescent labeling, RT-PCR and western blot demonstrated that hypoxia and ASIC3 over-expression inhibited the proliferation, arrested cell cycle in G1 phase, promoted the apoptosis, initiated the autophagy and up-regulated the expression of ASIC3, HIF-1α, light chain 3, p-ERK1/2 and p-MAPK. However, ASIC3 silencing could significantly relieve these phenomena. Co-immunoprecipitation assay found ASIC3 was interacted with HIF-1α&ERK1/2. Evaluation of the effect of HIF-1αsilencing on ASIC3 expression showed that the high expression of ASIC3 induced by hypoxia was reduced significantly by HIF-1α silencing. In conclusion, hypoxia and ASIC3 changed the behavior of nucleus pulposus cells by activating the MAPK pathway. HIF-1α and ASIC3 could regulate each other in nucleus pulposus cells." @default.
• W2952276039 created "2019-06-27" @default.
• W2952276039 creator A5009863789 @default.
• W2952276039 creator A5013120629 @default.
• W2952276039 creator A5033542590 @default.
• W2952276039 creator A5046943571 @default.
• W2952276039 creator A5056848219 @default.
• W2952276039 creator A5065636474 @default.
• W2952276039 date "2019-09-01" @default.
• W2952276039 modified "2023-10-01" @default.
• W2952276039 title "Effects of hypoxia and ASIC3 on nucleus pulposus cells: From cell behavior to molecular mechanism" @default.
• W2952276039 cites W1485297825 @default.
• W2952276039 cites W1986109746 @default.
• W2952276039 cites W1989892130 @default.
• W2952276039 cites W2023233675 @default.
• W2952276039 cites W2029449142 @default.
• W2952276039 cites W2032990871 @default.
• W2952276039 cites W2035908888 @default.
• W2952276039 cites W2039255471 @default.
• W2952276039 cites W2042594528 @default.
• W2952276039 cites W2046782983 @default.
• W2952276039 cites W2051915328 @default.
• W2952276039 cites W2053370953 @default.
• W2952276039 cites W2062939940 @default.
• W2952276039 cites W2065259405 @default.
• W2952276039 cites W2077906999 @default.
• W2952276039 cites W2161056273 @default.
• W2952276039 cites W2225487457 @default.
• W2952276039 cites W2231303906 @default.
• W2952276039 cites W2289095651 @default.
• W2952276039 cites W2399359399 @default.
• W2952276039 cites W2461828678 @default.
• W2952276039 cites W2465367340 @default.
• W2952276039 cites W2499628104 @default.
• W2952276039 cites W2567412918 @default.
• W2952276039 cites W2615198789 @default.
• W2952276039 cites W2626449510 @default.
• W2952276039 cites W2740728481 @default.
• W2952276039 cites W2743341503 @default.
• W2952276039 cites W2792424835 @default.
• W2952276039 cites W2794211319 @default.
• W2952276039 cites W2886791585 @default.
• W2952276039 cites W2888541448 @default.
• W2952276039 doi "https://doi.org/10.1016/j.biopha.2019.109061" @default.
• W2952276039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31202172" @default.
• W2952276039 hasPublicationYear "2019" @default.
• W2952276039 type Work @default.
• W2952276039 sameAs 2952276039 @default.
• W2952276039 citedByCount "10" @default.
• W2952276039 countsByYear W29522760392019 @default.
• W2952276039 countsByYear W29522760392021 @default.
• W2952276039 countsByYear W29522760392022 @default.
• W2952276039 countsByYear W29522760392023 @default.
• W2952276039 crossrefType "journal-article" @default.
• W2952276039 hasAuthorship W2952276039A5009863789 @default.
• W2952276039 hasAuthorship W2952276039A5013120629 @default.
• W2952276039 hasAuthorship W2952276039A5033542590 @default.
• W2952276039 hasAuthorship W2952276039A5046943571 @default.
• W2952276039 hasAuthorship W2952276039A5056848219 @default.
• W2952276039 hasAuthorship W2952276039A5065636474 @default.
• W2952276039 hasBestOaLocation W29522760391 @default.
• W2952276039 hasConcept C104317684 @default.
• W2952276039 hasConcept C119056186 @default.
• W2952276039 hasConcept C1491633281 @default.
• W2952276039 hasConcept C153911025 @default.
• W2952276039 hasConcept C173396325 @default.
• W2952276039 hasConcept C185592680 @default.
• W2952276039 hasConcept C190232843 @default.
• W2952276039 hasConcept C190283241 @default.
• W2952276039 hasConcept C2776415932 @default.
• W2952276039 hasConcept C54009773 @default.
• W2952276039 hasConcept C55493867 @default.
• W2952276039 hasConcept C57074206 @default.
• W2952276039 hasConcept C62478195 @default.
• W2952276039 hasConcept C86803240 @default.
• W2952276039 hasConcept C95444343 @default.
• W2952276039 hasConceptScore W2952276039C104317684 @default.
• W2952276039 hasConceptScore W2952276039C119056186 @default.
• W2952276039 hasConceptScore W2952276039C1491633281 @default.
• W2952276039 hasConceptScore W2952276039C153911025 @default.
• W2952276039 hasConceptScore W2952276039C173396325 @default.
• W2952276039 hasConceptScore W2952276039C185592680 @default.
• W2952276039 hasConceptScore W2952276039C190232843 @default.
• W2952276039 hasConceptScore W2952276039C190283241 @default.
• W2952276039 hasConceptScore W2952276039C2776415932 @default.
• W2952276039 hasConceptScore W2952276039C54009773 @default.
• W2952276039 hasConceptScore W2952276039C55493867 @default.
• W2952276039 hasConceptScore W2952276039C57074206 @default.
• W2952276039 hasConceptScore W2952276039C62478195 @default.
• W2952276039 hasConceptScore W2952276039C86803240 @default.
• W2952276039 hasConceptScore W2952276039C95444343 @default.
• W2952276039 hasLocation W29522760391 @default.
• W2952276039 hasLocation W29522760392 @default.
• W2952276039 hasOpenAccess W2952276039 @default.
• W2952276039 hasPrimaryLocation W29522760391 @default.
• W2952276039 hasRelatedWork W2003755164 @default.
• W2952276039 hasRelatedWork W2301659350 @default.
• W2952276039 hasRelatedWork W2349566129 @default.

• next