Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952324697> ?p ?o ?g. }
- W2952324697 abstract "Abstract High-throughput siRNA screens were only recently applied to cell factories to identify novel engineering targets which are able to boost cells towards desired phenotypes. While siRNA libraries exist for model organisms such as mice, no CHO-specific library is publicly available, hindering the application of this technique to CHO cells. The optimization of these cells is of special interest, as they are the main host for the production of therapeutic proteins. Here, we performed a cross-species approach by applying a mouse whole-genome siRNA library to CHO cells, optimized the protocol for suspension cultured cells, as this is the industrial practice for CHO cells, and developed an in silico method to identify functioning siRNAs, which also revealed the limitations of using cross-species libraries. With this method, we were able to identify several genes that, upon knockdown, enhanced the total productivity in the primary screen. A second screen validated two of these genes, Rad21 and Chd4 , whose knockdown was tested in additional CHO cell lines, confirming the induced high productivity phenotype, but also demonstrating the cell line/clone specificity of engineering effects." @default.
- W2952324697 created "2019-06-27" @default.
- W2952324697 creator A5001590359 @default.
- W2952324697 creator A5006303400 @default.
- W2952324697 creator A5018482539 @default.
- W2952324697 creator A5030780304 @default.
- W2952324697 creator A5041496905 @default.
- W2952324697 creator A5055349010 @default.
- W2952324697 creator A5060572919 @default.
- W2952324697 creator A5069201485 @default.
- W2952324697 creator A5078670673 @default.
- W2952324697 creator A5079466095 @default.
- W2952324697 creator A5079961784 @default.
- W2952324697 creator A5081715169 @default.
- W2952324697 creator A5083469226 @default.
- W2952324697 creator A5083911307 @default.
- W2952324697 creator A5084435777 @default.
- W2952324697 date "2019-06-18" @default.
- W2952324697 modified "2023-10-16" @default.
- W2952324697 title "A cross-species whole genome siRNA screen in suspension-cultured Chinese hamster ovary cells identifies novel engineering targets" @default.
- W2952324697 cites W1647075334 @default.
- W2952324697 cites W1830102282 @default.
- W2952324697 cites W1841746101 @default.
- W2952324697 cites W1889987954 @default.
- W2952324697 cites W1967734782 @default.
- W2952324697 cites W1971963900 @default.
- W2952324697 cites W1978403430 @default.
- W2952324697 cites W1991877884 @default.
- W2952324697 cites W1992973704 @default.
- W2952324697 cites W1998206066 @default.
- W2952324697 cites W2002044784 @default.
- W2952324697 cites W2017642141 @default.
- W2952324697 cites W2020084014 @default.
- W2952324697 cites W2029756273 @default.
- W2952324697 cites W2035393182 @default.
- W2952324697 cites W2047005748 @default.
- W2952324697 cites W2053437887 @default.
- W2952324697 cites W2068926449 @default.
- W2952324697 cites W2069942860 @default.
- W2952324697 cites W2072880634 @default.
- W2952324697 cites W2073412042 @default.
- W2952324697 cites W2093343938 @default.
- W2952324697 cites W2107277218 @default.
- W2952324697 cites W2110691960 @default.
- W2952324697 cites W2111054613 @default.
- W2952324697 cites W2113690540 @default.
- W2952324697 cites W2123752216 @default.
- W2952324697 cites W2124985265 @default.
- W2952324697 cites W2127173397 @default.
- W2952324697 cites W2128491398 @default.
- W2952324697 cites W2130005269 @default.
- W2952324697 cites W2131566517 @default.
- W2952324697 cites W2133003674 @default.
- W2952324697 cites W2136368512 @default.
- W2952324697 cites W2142850961 @default.
- W2952324697 cites W2158410771 @default.
- W2952324697 cites W2159267296 @default.
- W2952324697 cites W2168813197 @default.
- W2952324697 cites W2192080449 @default.
- W2952324697 cites W2317897504 @default.
- W2952324697 cites W2395509873 @default.
- W2952324697 cites W2398293253 @default.
- W2952324697 cites W2401135525 @default.
- W2952324697 cites W2559588208 @default.
- W2952324697 cites W2566325029 @default.
- W2952324697 cites W2591436500 @default.
- W2952324697 cites W2606661848 @default.
- W2952324697 cites W2753707914 @default.
- W2952324697 cites W2760954512 @default.
- W2952324697 cites W2790614447 @default.
- W2952324697 cites W2799830846 @default.
- W2952324697 cites W2800755544 @default.
- W2952324697 cites W2804667211 @default.
- W2952324697 cites W2806198647 @default.
- W2952324697 cites W2902516983 @default.
- W2952324697 cites W2917973694 @default.
- W2952324697 cites W2950636631 @default.
- W2952324697 doi "https://doi.org/10.1038/s41598-019-45159-2" @default.
- W2952324697 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6582146" @default.
- W2952324697 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31213643" @default.
- W2952324697 hasPublicationYear "2019" @default.
- W2952324697 type Work @default.
- W2952324697 sameAs 2952324697 @default.
- W2952324697 citedByCount "19" @default.
- W2952324697 countsByYear W29523246972019 @default.
- W2952324697 countsByYear W29523246972020 @default.
- W2952324697 countsByYear W29523246972021 @default.
- W2952324697 countsByYear W29523246972022 @default.
- W2952324697 countsByYear W29523246972023 @default.
- W2952324697 crossrefType "journal-article" @default.
- W2952324697 hasAuthorship W2952324697A5001590359 @default.
- W2952324697 hasAuthorship W2952324697A5006303400 @default.
- W2952324697 hasAuthorship W2952324697A5018482539 @default.
- W2952324697 hasAuthorship W2952324697A5030780304 @default.
- W2952324697 hasAuthorship W2952324697A5041496905 @default.
- W2952324697 hasAuthorship W2952324697A5055349010 @default.
- W2952324697 hasAuthorship W2952324697A5060572919 @default.
- W2952324697 hasAuthorship W2952324697A5069201485 @default.
- W2952324697 hasAuthorship W2952324697A5078670673 @default.
- W2952324697 hasAuthorship W2952324697A5079466095 @default.