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- W2952331750 abstract "Magnetotactic bacteria (MTB) are a diverse group of microorganisms capable of using geomagnetic fields for navigation. This magnetotactic behavior can help microorganisms move towards favorable habitats for optimal growth and reproduction. A comprehensive understanding of the magnetotactic mechanism at molecular levels requires highly efficient genomic editing tools, which remain underdeveloped in MTB. Here, we adapted an engineered CRISPR-Cas9 system for efficient inactivation of genes in a widely used MTB model strain, Magnetospirillum magneticum AMB-1. By combining a nuclease-deficient Cas9 (dCas9) and single-guide RNA (sgRNA), a CRISPR interference system was successfully developed to repress amb0994 expression. Furthermore, we constructed an in-frame deletion mutant of amb0994 by developing a CRISPR-Cas9 system. This mutant produces normal magnetosomes; however, its response to abrupt magnetic field reversals is faster than wild-type strain. This behavioral difference is probably a consequence of altered flagella function, as suggested with our dynamics simulation study by modeling M. magneticum AMB-1 cell as an ellipsoid. These data indicate that, Amb0994 is involved in the cellular response to magnetic torque changes via controlling flagella. In summary, this study, besides contributing to a better understanding of magnetotaxis mechanism, demonstrated the CRISPR-(d)Cas9 system as a useful genetic tool for efficient genome editing in MTB." @default.
- W2952331750 created "2019-06-27" @default.
- W2952331750 creator A5004807199 @default.
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- W2952331750 date "2018-07-17" @default.
- W2952331750 modified "2023-09-27" @default.
- W2952331750 title "Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Cas9 System for Analyzing Magnetotactic Behavior" @default.
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- W2952331750 doi "https://doi.org/10.3389/fmicb.2018.01569" @default.
- W2952331750 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6056624" @default.
- W2952331750 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30065707" @default.
- W2952331750 hasPublicationYear "2018" @default.
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