FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952334185> ?p ?o ?g. }

• W2952334185 endingPage "19.2019" @default.
• W2952334185 startingPage "ENEURO.0114" @default.
• W2952334185 abstract "Microglia play a pivotal role in maintaining homeostasis of the CNS. There is growing interest in understanding how microglia influence normal brain function and disease progression. Several microglia-specific Cx3cr1-Cre lines have been developed and have become indispensable tools in many investigations of microglial function. However, some recent studies have reported that these lines may have significant leakage into neurons. Other studies have reported that Cx3cr1 is expressed in non-microglial cells, including neurons and astrocytes, in vitro or in vivo either during brain development or upon neurological insult. All these reports raise serious concerns about the trustworthiness of these Cre-lines and whether the conclusions drawn from previous studies are valid. Here, we found that a floxed fluorescent reporter mouse line which has been frequently used to verify Cre lines displayed spontaneous expression of the GFP reporter, independent of Cre recombinase, thus revealing a potential caveat in assessing cre lines. We further confirmed that two Cx3cr1-Cre mouse lines can drive fluorescent reporter expression largely restrictively in microglia. Finally, we clarified that these two mouse lines maintain microglia-specific expression even following excitatory injury. Together, our findings confirm that two previously created Cx3cr1-Cre lines remain as invaluable tools for studying microglia. Moreover, to ensure the quality of data generated and the soundness of conclusions drawn from such data, it should be compulsory to thoroughly examine reporter lines for spontaneous leakiness when labeling cells to study CNS function and diseases." @default.
• W2952334185 created "2019-06-27" @default.
• W2952334185 creator A5015064413 @default.
• W2952334185 creator A5023913397 @default.
• W2952334185 creator A5035580389 @default.
• W2952334185 creator A5052731157 @default.
• W2952334185 creator A5054609222 @default.
• W2952334185 creator A5063087805 @default.
• W2952334185 creator A5066557906 @default.
• W2952334185 date "2019-06-14" @default.
• W2952334185 modified "2023-10-16" @default.
• W2952334185 title "Targeting Microglia Using Cx3cr1-Cre Lines: Revisiting the Specificity" @default.
• W2952334185 cites W1987332330 @default.
• W2952334185 cites W2000909573 @default.
• W2952334185 cites W2015776364 @default.
• W2952334185 cites W2017400430 @default.
• W2952334185 cites W2071832924 @default.
• W2952334185 cites W2082977820 @default.
• W2952334185 cites W2096500024 @default.
• W2952334185 cites W2103946141 @default.
• W2952334185 cites W2117360135 @default.
• W2952334185 cites W2125954178 @default.
• W2952334185 cites W2146316688 @default.
• W2952334185 cites W2153406260 @default.
• W2952334185 cites W2161057248 @default.
• W2952334185 cites W2183197284 @default.
• W2952334185 cites W2469773095 @default.
• W2952334185 cites W2755381547 @default.
• W2952334185 cites W2782347637 @default.
• W2952334185 cites W2788589260 @default.
• W2952334185 cites W2790335044 @default.
• W2952334185 cites W2804756252 @default.
• W2952334185 cites W2887447835 @default.
• W2952334185 cites W576141130 @default.
• W2952334185 doi "https://doi.org/10.1523/eneuro.0114-19.2019" @default.
• W2952334185 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6620394" @default.
• W2952334185 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31201215" @default.
• W2952334185 hasPublicationYear "2019" @default.
• W2952334185 type Work @default.
• W2952334185 sameAs 2952334185 @default.
• W2952334185 citedByCount "46" @default.
• W2952334185 countsByYear W29523341852019 @default.
• W2952334185 countsByYear W29523341852020 @default.
• W2952334185 countsByYear W29523341852021 @default.
• W2952334185 countsByYear W29523341852022 @default.
• W2952334185 countsByYear W29523341852023 @default.
• W2952334185 crossrefType "journal-article" @default.
• W2952334185 hasAuthorship W2952334185A5015064413 @default.
• W2952334185 hasAuthorship W2952334185A5023913397 @default.
• W2952334185 hasAuthorship W2952334185A5035580389 @default.
• W2952334185 hasAuthorship W2952334185A5052731157 @default.
• W2952334185 hasAuthorship W2952334185A5054609222 @default.
• W2952334185 hasAuthorship W2952334185A5063087805 @default.
• W2952334185 hasAuthorship W2952334185A5066557906 @default.
• W2952334185 hasBestOaLocation W29523341851 @default.
• W2952334185 hasConcept C102230213 @default.
• W2952334185 hasConcept C104317684 @default.
• W2952334185 hasConcept C125929170 @default.
• W2952334185 hasConcept C12823836 @default.
• W2952334185 hasConcept C13373296 @default.
• W2952334185 hasConcept C141035611 @default.
• W2952334185 hasConcept C142613039 @default.
• W2952334185 hasConcept C169760540 @default.
• W2952334185 hasConcept C203014093 @default.
• W2952334185 hasConcept C207001950 @default.
• W2952334185 hasConcept C2776914184 @default.
• W2952334185 hasConcept C2779830541 @default.
• W2952334185 hasConcept C31179232 @default.
• W2952334185 hasConcept C54355233 @default.
• W2952334185 hasConcept C81885089 @default.
• W2952334185 hasConcept C86803240 @default.
• W2952334185 hasConcept C95444343 @default.
• W2952334185 hasConceptScore W2952334185C102230213 @default.
• W2952334185 hasConceptScore W2952334185C104317684 @default.
• W2952334185 hasConceptScore W2952334185C125929170 @default.
• W2952334185 hasConceptScore W2952334185C12823836 @default.
• W2952334185 hasConceptScore W2952334185C13373296 @default.
• W2952334185 hasConceptScore W2952334185C141035611 @default.
• W2952334185 hasConceptScore W2952334185C142613039 @default.
• W2952334185 hasConceptScore W2952334185C169760540 @default.
• W2952334185 hasConceptScore W2952334185C203014093 @default.
• W2952334185 hasConceptScore W2952334185C207001950 @default.
• W2952334185 hasConceptScore W2952334185C2776914184 @default.
• W2952334185 hasConceptScore W2952334185C2779830541 @default.
• W2952334185 hasConceptScore W2952334185C31179232 @default.
• W2952334185 hasConceptScore W2952334185C54355233 @default.
• W2952334185 hasConceptScore W2952334185C81885089 @default.
• W2952334185 hasConceptScore W2952334185C86803240 @default.
• W2952334185 hasConceptScore W2952334185C95444343 @default.
• W2952334185 hasFunder F4320337357 @default.
• W2952334185 hasFunder F4320337359 @default.
• W2952334185 hasIssue "4" @default.
• W2952334185 hasLocation W29523341851 @default.
• W2952334185 hasLocation W29523341852 @default.
• W2952334185 hasLocation W29523341853 @default.
• W2952334185 hasLocation W29523341854 @default.
• W2952334185 hasOpenAccess W2952334185 @default.
• W2952334185 hasPrimaryLocation W29523341851 @default.

• next