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- W2952373279 abstract "ABSTRACT MicroED (micro electron diffraction) is an emerging technique to use cryo-electron microscope to study the crystal structure of macromolecule from its micro/nano-crystals, which are not suitable for conventional X-ray crystallography. However, this technique has been prevented for its wide application by the limited availability of producing good micro-/nano-crystals and the inappropriate transfer of crystals. Here, we developed a complete workflow to prepare suitable crystals efficiently for MicroED experiment. This workflow includes in situ on-grid crystallization, single-side blotting, cryo-focus ion beam (cryo-FIB) fabrication, and cryo-electron diffraction of crystal cryo-lamella. This workflow enables us to apply MicroED to study many small macromolecular crystals with the size of 2 ~ 10 μm, which is too large for MicroED but quite small for conventional X-ray crystallography. We have applied this method to solve 2.5Å crystal structure of lysozyme from its micro-crystal within the size of 10×10×10 μm 3 . Our work will greatly expand the availability space of crystals suitable for MicroED and fill up the gap between MicroED and X-ray crystallography." @default.
- W2952373279 created "2019-06-27" @default.
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- W2952373279 date "2018-07-25" @default.
- W2952373279 modified "2023-10-17" @default.
- W2952373279 title "In situ protein micro-crystal fabrication by cryo-FIB for electron diffraction" @default.
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- W2952373279 doi "https://doi.org/10.1101/377192" @default.
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