FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952380592> ?p ?o ?g. }

• W2952380592 endingPage "10" @default.
• W2952380592 startingPage "1" @default.
• W2952380592 abstract "Ischemia/reperfusion- (I/R-) induced organ damage represents one of the main causes of death worldwide, and new strategies to reduce I/R injury are urgently needed. We have shown that programmable cells of monocytic origin (PCMO) respond to I/R with the release of angiogenic mediators and that transplantation of PCMO results in increased neovascularization. Human regulatory macrophages (Mreg), which are also of monocytic origin, have been successfully employed in clinical transplantation studies due to their immunomodulatory properties. Here, we investigated whether Mreg also possess angiogenic potential in vitro and could represent a treatment option for I/R-associated illnesses. Mreg were differentiated using peripheral blood monocytes from different donors (N = 14) by incubation with M-CSF and human AB serum and stimulation with INF-gamma. Mreg cultures were subjected to 3 h of hypoxia and 24 h of reoxygenation (resembling I/R) or the respective nonischemic control. Cellular resilience, expression of pluripotency markers, secretion of angiogenic proteins, and influence on endothelial tube formation as a surrogate marker for angiogenesis were investigated. Mreg showed resilience against I/R that did not lead to increased cell damage. Mreg express DHRS9 as well as IDO and display a moderate to low expression pattern of several pluripotency genes (e.g., NANOG, OCT-4, and SOX2). I/R resulted in an upregulation of IDO (p < 0.001) while C-MYC and KLF4 were downregulated (p < 0.001 and p < 0.05). Proteome profiling revealed the secretion of numerous angiogenic proteins by Mreg of which several were strongly upregulated by I/R (e.g., MIP-1alpha, 19.9-fold; GM-CSF, 19.2-fold; PTX3, 5.8-fold; IL-1β, 5.2-fold; and MCP-1, 4.7-fold). The angiogenic potential of supernatants from Mreg subjected to I/R remains inconclusive. While Mreg supernatants from 3 donors induced tube formation, 2 supernatants were not effective. We suggest that Mreg may prove beneficial as a cell therapy-based treatment option for I/R-associated illnesses. However, donor characteristics seem to crucially influence the effectiveness of Mreg treatment." @default.
• W2952380592 created "2019-06-27" @default.
• W2952380592 creator A5004375284 @default.
• W2952380592 creator A5009433799 @default.
• W2952380592 creator A5015146575 @default.
• W2952380592 creator A5019488739 @default.
• W2952380592 creator A5034281218 @default.
• W2952380592 creator A5042405615 @default.
• W2952380592 creator A5048056231 @default.
• W2952380592 creator A5050537449 @default.
• W2952380592 creator A5068893443 @default.
• W2952380592 date "2019-06-25" @default.
• W2952380592 modified "2023-10-17" @default.
• W2952380592 title "Characterization of the Angiogenic Potential of Human Regulatory Macrophages (Mreg) after Ischemia/Reperfusion Injury In Vitro" @default.
• W2952380592 cites W1516197123 @default.
• W2952380592 cites W1533804294 @default.
• W2952380592 cites W1922808040 @default.
• W2952380592 cites W1973316957 @default.
• W2952380592 cites W1993401937 @default.
• W2952380592 cites W1993804011 @default.
• W2952380592 cites W2019136342 @default.
• W2952380592 cites W2020375403 @default.
• W2952380592 cites W2026100254 @default.
• W2952380592 cites W2030828007 @default.
• W2952380592 cites W2033605309 @default.
• W2952380592 cites W2035352210 @default.
• W2952380592 cites W2045184816 @default.
• W2952380592 cites W2047463251 @default.
• W2952380592 cites W2050841260 @default.
• W2952380592 cites W2060911356 @default.
• W2952380592 cites W2081778817 @default.
• W2952380592 cites W2095598251 @default.
• W2952380592 cites W2100190333 @default.
• W2952380592 cites W2108790161 @default.
• W2952380592 cites W2109450293 @default.
• W2952380592 cites W2114190644 @default.
• W2952380592 cites W2117603154 @default.
• W2952380592 cites W2119203970 @default.
• W2952380592 cites W2127845947 @default.
• W2952380592 cites W2131065702 @default.
• W2952380592 cites W2152792998 @default.
• W2952380592 cites W2156393231 @default.
• W2952380592 cites W2257472842 @default.
• W2952380592 cites W2512289339 @default.
• W2952380592 cites W2552499478 @default.
• W2952380592 cites W2553602297 @default.
• W2952380592 cites W2621358572 @default.
• W2952380592 cites W2623293779 @default.
• W2952380592 cites W2746710261 @default.
• W2952380592 cites W2784299734 @default.
• W2952380592 cites W2804835621 @default.
• W2952380592 cites W2882980351 @default.
• W2952380592 cites W2884173452 @default.
• W2952380592 cites W2900777808 @default.
• W2952380592 cites W4293084779 @default.
• W2952380592 doi "https://doi.org/10.1155/2019/3725863" @default.
• W2952380592 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6614961" @default.
• W2952380592 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31341483" @default.
• W2952380592 hasPublicationYear "2019" @default.
• W2952380592 type Work @default.
• W2952380592 sameAs 2952380592 @default.
• W2952380592 citedByCount "11" @default.
• W2952380592 countsByYear W29523805922020 @default.
• W2952380592 countsByYear W29523805922021 @default.
• W2952380592 countsByYear W29523805922023 @default.
• W2952380592 crossrefType "journal-article" @default.
• W2952380592 hasAuthorship W2952380592A5004375284 @default.
• W2952380592 hasAuthorship W2952380592A5009433799 @default.
• W2952380592 hasAuthorship W2952380592A5015146575 @default.
• W2952380592 hasAuthorship W2952380592A5019488739 @default.
• W2952380592 hasAuthorship W2952380592A5034281218 @default.
• W2952380592 hasAuthorship W2952380592A5042405615 @default.
• W2952380592 hasAuthorship W2952380592A5048056231 @default.
• W2952380592 hasAuthorship W2952380592A5050537449 @default.
• W2952380592 hasAuthorship W2952380592A5068893443 @default.
• W2952380592 hasBestOaLocation W29523805921 @default.
• W2952380592 hasConcept C104317684 @default.
• W2952380592 hasConcept C126322002 @default.
• W2952380592 hasConcept C142724271 @default.
• W2952380592 hasConcept C203014093 @default.
• W2952380592 hasConcept C2777933648 @default.
• W2952380592 hasConcept C2778265714 @default.
• W2952380592 hasConcept C2780114680 @default.
• W2952380592 hasConcept C2780394083 @default.
• W2952380592 hasConcept C2911091166 @default.
• W2952380592 hasConcept C502942594 @default.
• W2952380592 hasConcept C541997718 @default.
• W2952380592 hasConcept C54355233 @default.
• W2952380592 hasConcept C71924100 @default.
• W2952380592 hasConcept C86339819 @default.
• W2952380592 hasConcept C86803240 @default.
• W2952380592 hasConcept C95444343 @default.
• W2952380592 hasConceptScore W2952380592C104317684 @default.
• W2952380592 hasConceptScore W2952380592C126322002 @default.
• W2952380592 hasConceptScore W2952380592C142724271 @default.
• W2952380592 hasConceptScore W2952380592C203014093 @default.
• W2952380592 hasConceptScore W2952380592C2777933648 @default.
• W2952380592 hasConceptScore W2952380592C2778265714 @default.

• next