Matches in SemOpenAlex for { <https://semopenalex.org/work/W2952425397> ?p ?o ?g. }
- W2952425397 abstract "Genetically encoded calcium ion (Ca2+) indicators (GECIs) are indispensable tools for measuring Ca2+ dynamics and neuronal activities in vitro and in vivo. Red fluorescent protein (RFP)-based GECIs have inherent advantages relative to green fluorescent protein-based GECIs due to the longer wavelength light used for excitation. Longer wavelength light is associated with decreased phototoxicity and deeper penetration through tissue. Red GECI can also enable multicolor visualization with blue- or cyan-excitable fluorophores. Here we report the development, structure, and validation of a new RFP-based GECI, K-GECO1, based on a circularly permutated RFP derived from the sea anemone Entacmaea quadricolor. We have characterized the performance of K-GECO1 in cultured HeLa cells, dissociated neurons, stem-cell-derived cardiomyocytes, organotypic brain slices, zebrafish spinal cord in vivo, and mouse brain in vivo. K-GECO1 is the archetype of a new lineage of GECIs based on the RFP eqFP578 scaffold. It offers high sensitivity and fast kinetics, similar or better than those of current state-of-the-art indicators, with diminished lysosomal accumulation and minimal blue-light photoactivation. Further refinements of the K-GECO1 lineage could lead to further improved variants with overall performance that exceeds that of the most highly optimized red GECIs." @default.
- W2952425397 created "2019-06-27" @default.
- W2952425397 creator A5005601864 @default.
- W2952425397 creator A5008382439 @default.
- W2952425397 creator A5019877657 @default.
- W2952425397 creator A5020940486 @default.
- W2952425397 creator A5027042656 @default.
- W2952425397 creator A5045866054 @default.
- W2952425397 creator A5052816130 @default.
- W2952425397 creator A5054437859 @default.
- W2952425397 creator A5056166073 @default.
- W2952425397 creator A5057156357 @default.
- W2952425397 creator A5058624570 @default.
- W2952425397 creator A5065727681 @default.
- W2952425397 creator A5070170043 @default.
- W2952425397 creator A5074910375 @default.
- W2952425397 creator A5077107149 @default.
- W2952425397 creator A5077797936 @default.
- W2952425397 creator A5078236144 @default.
- W2952425397 creator A5082139232 @default.
- W2952425397 creator A5084176336 @default.
- W2952425397 creator A5088191467 @default.
- W2952425397 creator A5089262525 @default.
- W2952425397 date "2018-01-16" @default.
- W2952425397 modified "2023-10-10" @default.
- W2952425397 title "A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578" @default.
- W2952425397 cites W1480802716 @default.
- W2952425397 cites W1539796472 @default.
- W2952425397 cites W1557879301 @default.
- W2952425397 cites W1571164133 @default.
- W2952425397 cites W1967305385 @default.
- W2952425397 cites W1967626349 @default.
- W2952425397 cites W1967642375 @default.
- W2952425397 cites W1968320713 @default.
- W2952425397 cites W1972289308 @default.
- W2952425397 cites W1977643725 @default.
- W2952425397 cites W1980338953 @default.
- W2952425397 cites W1984214088 @default.
- W2952425397 cites W1990076574 @default.
- W2952425397 cites W1992875306 @default.
- W2952425397 cites W2007981920 @default.
- W2952425397 cites W2013151879 @default.
- W2952425397 cites W2013613467 @default.
- W2952425397 cites W2017989927 @default.
- W2952425397 cites W2020852375 @default.
- W2952425397 cites W2039409076 @default.
- W2952425397 cites W2044546215 @default.
- W2952425397 cites W2050044028 @default.
- W2952425397 cites W2052344357 @default.
- W2952425397 cites W2054344135 @default.
- W2952425397 cites W2055725584 @default.
- W2952425397 cites W2058197627 @default.
- W2952425397 cites W2059536603 @default.
- W2952425397 cites W2067492027 @default.
- W2952425397 cites W2073046174 @default.
- W2952425397 cites W2080092859 @default.
- W2952425397 cites W2099540110 @default.
- W2952425397 cites W2106246537 @default.
- W2952425397 cites W2106355804 @default.
- W2952425397 cites W2107913200 @default.
- W2952425397 cites W2108921801 @default.
- W2952425397 cites W2109234881 @default.
- W2952425397 cites W2115734610 @default.
- W2952425397 cites W2115792839 @default.
- W2952425397 cites W2122683100 @default.
- W2952425397 cites W2124026197 @default.
- W2952425397 cites W2125575640 @default.
- W2952425397 cites W2126672460 @default.
- W2952425397 cites W2133777156 @default.
- W2952425397 cites W2144147853 @default.
- W2952425397 cites W2150534859 @default.
- W2952425397 cites W2152018227 @default.
- W2952425397 cites W2157199084 @default.
- W2952425397 cites W2159132385 @default.
- W2952425397 cites W2160919229 @default.
- W2952425397 cites W2163341755 @default.
- W2952425397 cites W2166357485 @default.
- W2952425397 cites W2167095271 @default.
- W2952425397 cites W2171332611 @default.
- W2952425397 cites W2283116424 @default.
- W2952425397 cites W2291957660 @default.
- W2952425397 cites W2301795778 @default.
- W2952425397 cites W2567050542 @default.
- W2952425397 cites W2587514184 @default.
- W2952425397 cites W2593007003 @default.
- W2952425397 cites W2650870900 @default.
- W2952425397 doi "https://doi.org/10.1186/s12915-018-0480-0" @default.
- W2952425397 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5771076" @default.
- W2952425397 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29338710" @default.
- W2952425397 hasPublicationYear "2018" @default.
- W2952425397 type Work @default.
- W2952425397 sameAs 2952425397 @default.
- W2952425397 citedByCount "79" @default.
- W2952425397 countsByYear W29524253972018 @default.
- W2952425397 countsByYear W29524253972019 @default.
- W2952425397 countsByYear W29524253972020 @default.
- W2952425397 countsByYear W29524253972021 @default.
- W2952425397 countsByYear W29524253972022 @default.
- W2952425397 countsByYear W29524253972023 @default.
- W2952425397 crossrefType "journal-article" @default.