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- W2952432076 abstract "Although the role of 5-methylcytosine has been well studied, the biological role of 5-hydroxymethylcytosine still remains unclear because of the limited methods available for single-base detection of 5-hydroxymethylcytosine (5hmC). Here, we present mirror bisulfite sequencing for 5hmC detection at a single CpG site by synthesizing a DNA strand to mirror the parental strand. This semiconservative duplex is sequentially treated with β-glucosyltransferase and M.SssI methylase. The glucosyl-5hmCpG in the parental strand inhibits methylation of the mirroring CpG site, and after bisulfite conversion, a thymine in the mirroring strand indicates a 5hmCpG site in the parental strand, whereas a cytosine indicates a non-5hmC site. Using this method, the 5hmC levels of various human tissues and paired liver tissues were mapped genomewide." @default.
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- W2952432076 date "2018-10-22" @default.
- W2952432076 modified "2023-09-25" @default.
- W2952432076 title "Mirror Bisulfite Sequencing: A Method for Single-Base Resolution of Hydroxymethylcytosine" @default.
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- W2952432076 doi "https://doi.org/10.1021/acs.analchem.8b02832" @default.
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